江西农业大学学报2016，Vol.38Issue(4)：646-653,8.DOI:10.13836/j.jjau.2016092
杉木杂交育种中亲本选配的方法研究
Methods for Parent Apolegamy of Chinese-fir in Cross Breeding
摘要
Abstract
Cross breeding is the main approach to genetic breeding of Chinese fir.In order to match cross parents reasonably,growth data of 12 cross combinations and 4 inbreeding combinations were used for study. The genotypes of the Chinese fir parents were obtained through the allozyme analysis.The photosynthetic rate of the parents of Chinese⁃fir at fast⁃growing period was measured by an indirect procedure.The average respiration rate of the parents was determined by infrared CO2 respirometer(GHX⁃3051H).On the basis of the study of the allozyme diversity of the parents,heterotic grouping method ( HGM) and forecasting model method ( FMM) were adopted respectively to study the parents’ apolegamy in crossing breeding of Chinese⁃fir.HGM depended on the results of the cluster analysis of parents allozyme information,then it was combined with the research re⁃sults of the Chinese fir cytogenetics,selecting the parent with higher photosynthetic rate as female;selecting the parent with higher respiratory rate as male.FMM was carried out on the parents’ apolegamy based on forecas⁃ting the average performance of the cross combinations.Firstly,using allozyme information and physiological in⁃dex relating to the volume growth,multiple regression models were established to predict the average perform⁃ance of the cross combinations and then the parent to be used as female or male could be confirmed.The accu⁃racy of these models was verified by the growth data of five 10⁃year⁃old cross combinations.Validation of the models reliability is feasible.The results of the two methods between HGM and FMM are mutually interdepend⁃ent,it is more effective that two methods should be used together.Therefore the HGM and FMM have applica⁃tion values in the practice of cross breeding of Chinese fir.关键词
杉木/亲本选配/等位酶/母本细胞质效应/杂种优势群法/预测模型法Key words
Cunninghamia lanceolata Hook./parent apolegamy/allozyme/female cytoplasm effects/HGM/FMM分类
农业科技引用本文复制引用
何贵平,齐明,程亚平,徐肇友,罗修宝,何必庭..杉木杂交育种中亲本选配的方法研究[J].江西农业大学学报,2016,38(4):646-653,8.基金项目
浙江省“十二五”竹木育种专项（2012C12908-11）和国家“十二五”科技支撑项目（2012BAD01B0201） Project supportted by Bamboo and Wood Breeding Special Subjects of Zhejiang Province “ Twelve-five ”（2012C12908-11） and Projects of State Science and Technology in “Twelve-five” （）
