江西农业大学学报2016，Vol.38Issue(4)：741-746,6.DOI:10.13836/j.jjau.2016106
生殖生长期烟草 orf25基因的表达和 ATP 酶活性分析
An Analysis of orf25 Gene and ATPase Activtity of Tobacco during Reproduction Stage
摘要
Abstract
To investigate the relationship between the orf25 gene and male sterility in tobacco,the (CMS) Gexin 3 and Gexin 3,which were in the reproductive growth stage,were used as experimental materials.The re⁃al⁃time quantitative PCR(qRT⁃PCR) was used to analyze the expression patterns of the orf25 gene,and the activity of ATPase was also tested.The results showed that the expression amount of the orf25 gene in Gexin 3 was relatively higher than that in(CMS)Gexin 3,furthermore,the expression of the orf25 gene in flowers was the highest in the same material,that in the leaves took the second place,the expression of orf25 gene in roots was relatively low.The activity of ATPase in the maintainer line was relatively higher than that in its male sterile line,the activity of the ATPase in the flowers was also the highest in the same materials.There was no significant difference of ATPase in leaves and roots.The correlation analysis indicated that the relative expression of orf25 gene had extremely significant positive correlation with the activity of ATPase in the same organ of tobacco.关键词
烟草/orf25 基因/ATP 酶/雄性不育Key words
tobacco/orf25 gene/ATPase/cytoplasmic male sterility分类
农业科技引用本文复制引用
吴凌敏,陶瑶,谢丽娟,聂亚平,钟思荣,朱肖文,刘齐元..生殖生长期烟草 orf25基因的表达和 ATP 酶活性分析[J].江西农业大学学报,2016,38(4):741-746,6.基金项目
国家自然科学基金项目（31260350）和江西省教育厅科技计划项目（GJJ13275） Project supported by the National Natural Science Foundation of China （31260350） and Science Planning Pro-ject of Jiangxi Provincial Education Department （）
