检验医学与临床2016，Vol.13Issue(17)：2455-2457,2460,4.DOI:10.3969/j.issn.1672-9455.2016.17.019
缺氧诱导因子-1α的表达在大鼠急性一氧化碳中毒迟发型脑病中的作用
The expression of hypoxia-inducible factor-1α in hippocampus of rats with delayed encephalopathy after acute carbon monoxide poisoning
余倩 1邓楠 1朱木林 1沈流燕 1周莉 1李经伦2
作者信息
- 1. 四川省泸州市人民医院神经内科 646000
- 2. 西南医科大学附属医院神经内科，四川泸州646099
- 折叠
摘要
Abstract
Objective To observe the effect of hypoxia‐inducible factor‐1 alpha(HIF‐1α) in the pathogenesis of delayed encepha‐lopathy after acute carbon monoxide poisoning(DEACMP) .Methods 90 male SD rats(250 -320 g) were randomly divided into three groups:blank control group(BC group) ,air control group(AC group) and DEACMP group .The time points of 1st ,3rd ,7th , 14th ,21th and 28th day after acute carbon monoxide poisoning were set for measuring the changes .The rats in DEACMP group were injected in abdominal cavity with pure CO several times to establish DEACMP model ,rats in AC group were injected with e‐qual air in the same way ,BC group was without any treatment .Morris water maze test was used to measure the learning ability and memory .The apoptosis of pyramidal neurons at hippocampal gyrus area was measured by TUNEL test .The expression of HIF‐1αin hippocampal area was measured by immunohistochemistry .Results The average escaped latency of rats in DEACMP group were increased after poisoning compared with rats in other two groups(P<0 .05) .The time during platform quadrant was shortened in CO group rats after poisoning(P<0 .05) .The apoptosis of pyramidal neurons in hippocampal gyrus was found increased since the 3rd day after the CO poisoning ,and it was reached the peak at the 7th day ,The apoptotic index in CO group was increased signifi‐cantly compare with BC group and AC group (P<0 .05) .The expression of HIF‐1αin hippocampal gyrus was found increased since the 1st day after the CO poisoning ,reached the peak at the 3rd day ,and it still had a high expression at 28th day .The expression in CO group was increased significantly compare with BC group and AC group(P<0 .05) .Conclusion HIF‐1αmay play an important role in DEACM P by inducing the apoptosis of neurons in hippocampal gyrus area .关键词
急性一氧化碳中毒迟发型脑病/细胞凋亡/缺氧诱导因子1αKey words
delayed encephalopathy after acute carbon monoxide poisoning/cell apoptosis/hypoxia-inducible factor-1α引用本文复制引用
余倩,邓楠,朱木林,沈流燕,周莉,李经伦..缺氧诱导因子-1α的表达在大鼠急性一氧化碳中毒迟发型脑病中的作用[J].检验医学与临床,2016,13(17):2455-2457,2460,4.
