口腔颌面外科杂志2016，Vol.26Issue(4)：244-250,7.DOI:10.3969/j.issn.1005-4979.2016.04.003
双膦酸盐对大鼠颌面骨及外周骨不同来源骨髓间充质干细胞成骨分化的影响
Effect of Bisphosphonates on Osteogenic Potential of Mandibular vs. Long-bone Mesenchymal Stem Cells in Rats
摘要
Abstract
Objective: To investigate the effects of zoledronate, a third generation of bisphosphonates commonly used in clinical practice,on the proliferation and osteogenic differentiation potential of mandibular and long-bone mesenchymal stem cells (BMSCs) in rats. As well as, to study the discrepancy of drug response to biological characteristics of cells derived from distinct skeletal sites. Methods: The BMSCs isolated from the mandible and ilium bone of SD newborn rats were cultured with different concentrations of zoledronate. At 3 days after zoledronate exposure, Alarmar Blue assay was used to measure the absorbance to determine the effect of zoledronate on the proliferation of mandible and ilium mesenchymal stem cells. Cell alkaline phosphatase activity and osteocalcin secretion of mandible and ilium BMSCs were analyzed. Results:Zoledronate at the concentration of ≥2.0 μg/mL could inhibit the proliferation of ilium BMSCs, but it had no effect at the concentration of ≤1.0 μg/mL. For mandible BMSCs, zoledronate at the concentration of ≤0.5 μg/mL inhibited the cell proliferation significantly. Zoledronate (1.0μg/mL) could also inhibit cell alkaline phosphatase activity, osteocalcin secretion of mandible BMSCs, but at the same concentration, it had no effect to ilium BMSCs. Conclusion:Our findings support that function and differentiation potential of BMSCs derived from distinct origins after exposure to zoledronate is discrepant, andprovide a new theoretical basis to clarify pathogenesis of bisphosphonates related osteonecrosis of the jaw (BRONJ).关键词
双膦酸盐/颌骨/髂骨/骨髓间充质干细胞/增殖/成骨分化Key words
bisphosphonates/mandible/ilium/bone mesenchymal stem cells/proliferation/osteogenic differentiation分类
医药卫生引用本文复制引用
俞宛璐,苏俭生..双膦酸盐对大鼠颌面骨及外周骨不同来源骨髓间充质干细胞成骨分化的影响[J].口腔颌面外科杂志,2016,26(4):244-250,7.基金项目
国家自然科学基金（81371949,81572114）；上海市科学技术委员会医学重点项目（13411951201）；教育部博士点基金 （）
