临床口腔医学杂志2016，Vol.32Issue(9)：515-518,519,5.DOI:10.3969/j.issn.1003-1634.2016.09.002
B7-1、B7-2在牙龈卟啉单胞菌脂多糖诱导的T淋巴细胞反应中的作用
Effect of B7-1 and B7-2 on T lymphocyte response induced by lipopolysaccharide of Porphyromonas gingivalis
摘要
Abstract
Objective:To investigate the effect of B7⁃1 and B7⁃2 on T lymphocyte response induced by lipopolysac⁃charide( LPS) of Porphyromonas gingivalis( P. g) . Methods:Dendritic cells( DCs) were cultured from the bone marrow of B7⁃1 KO,B7⁃2 KO,B7⁃1/2 KO and wild type( WT) mice. DCs were stimulated with LPS of P. g. in advance and then co⁃cul⁃tured with T lymphocytes. CCK8 kit was used to examine the proliferation of T lymphocytes. Cytokines such as IFN⁃γ,IL⁃4 and IL⁃17 in the supernatants were detected by ELISA. Quantitative real⁃time PCR( qPCR) was performed to test the RNA levels of IFN⁃γ,IL⁃4,IL⁃17,GATA⁃3,T⁃bet and RORγt of T cells. Results:There was no significant difference in T cell pro⁃liferation among groups. Compared with WT group,B7⁃1 KO group showed decreased IFN⁃γ and T⁃bet,increased IL⁃4 and GATA⁃3,and similar levels of IL⁃17 and RORγt. Whereas,B7⁃2 KO group showed higher levels of IFN⁃γ and T⁃bet,and lower levels of IL⁃4,GATA⁃3,IL⁃17 and RORγt than WT group. The variation tendency of these factors in B7⁃1/2 KO group was between those of B7⁃1 KO group and B7⁃2 KO group. Conclusion:Deficency of B7⁃1 and/or B7⁃2 in DCs does not in⁃fluence DCs⁃induced T lymphocyte proliferation. B7⁃1 may promote T lymphocyte differentiation to Th1 cells,inhibit its dif⁃ferentiation to Th2 cells,While B7⁃2 may inhibit T lymphocyte differentiation to Th1 cells and promote its differentiation to Th2 and Th17 cells.关键词
牙龈卟啉单胞菌/脂多糖/B7-1/B7-2/T淋巴细胞Key words
Porphyromonas gingivalis/Lipopolysaccharide/B7-1/B7-2/T lymphocytes分类
医药卫生引用本文复制引用
王莹,许庆安,樊明文..B7-1、B7-2在牙龈卟啉单胞菌脂多糖诱导的T淋巴细胞反应中的作用[J].临床口腔医学杂志,2016,32(9):515-518,519,5.基金项目
国家自然科学基金 （）
