临床口腔医学杂志2016，Vol.32Issue(9)：523-526,4.DOI:10.3969/j.issn.1003-1634.2016.09.004
DGGE分析中间普雷沃菌在原发性根尖周感染中的基因多态性
Genotypic analyses of Prevotella intermedia in primary endodontic infections by DGGE
摘要
Abstract
Objective: To analyze the genetic diversity of Prevotella intermedia in primary endodontic infections�Methods:Seventeen microbial samples were taken from the root canals of teeth with primary endodontic infec⁃tions�DNA extracted from the samples was amplified by nested PCR to detect P�intermedia�It was conducted with P�intermedia ⁃specific primers based on 16 S rDNA V3⁃V6 region,and several DGGE bands were excised for further sequen⁃cing�Results:The prevalence of P�intermediain primary endodontic infections was 47�06%�There were 14 DGGE bands present at different position�It was founded 3 genetic according to sequencing and blasting�Conclusion:Genetic diversity of P�intermedia was presented by PCR⁃DGGE.关键词
原发性根尖周炎/中间普雷沃菌/基因多态性/变性梯度凝胶电泳Key words
Primary endodontic infections/Prevotella intermedia/Genepolymorphism/Denatured gradient gel elec-trophoresis分类
医药卫生引用本文复制引用
邹岩,漆正楠,黄正蔚,何智妍,朱彩莲,唐子圣..DGGE分析中间普雷沃菌在原发性根尖周感染中的基因多态性[J].临床口腔医学杂志,2016,32(9):523-526,4.基金项目
上海市自然科学基金（13ZR1423900）；上海交通大学“医工（理）交叉研究”基金资助项目 （）
