临床输血与检验2016，Vol.18Issue(5)：424-426,427,4.DOI:10.3969/j.issn.1671-2587.2016.05.005
ABO亚型B（A）04的鉴定及分子机制研究
Identification of Serologic and Molecular Bases of B(A)04 Subtype of ABO Blood Group
摘要
Abstract
Objective To investigate the characters of B(A)04 subtype in order to provide a theoretical basis for the work of clinical blood transfusion. Methods The characteristics of B(A)04 subtype were identified by serological and genotyping technology. Exons 1-7 and adjacent introns of the ABO gene were amplified by polymerase chain reaction (PCR). The PCR products were directly sequenced and cloning sequenced to identify the molecular mechanism of B(A)04 subtype. Results The patient showed ABO forward and reverse typing discrepancy. ABO forward typing defined A weak B. However, A1 cells (2+) and B cells (-) were observed in the reverse typing. The ABO gene sequencing showed a A>G substitution at position in exon 7 which caused a Met to Val exchange at codon 214. The ABO blood group genotype was ABO*B(A)04/O01. Conclusion The identification and analysis of subordinate or rare blood types should be very careful. In order to ensure the safety of blood transfusion, the molecular biological detection should carried out when necessary.关键词
ABO血型/B(A)亚型/ABO基因/ABO基因分型Key words
ABO blood group/B(A) subgroup/ABO gene/ABO genotype分类
医药卫生引用本文复制引用
方乐,肖建宇,吴敏慧,叶燕,黄成垠,陈青..ABO亚型B（A）04的鉴定及分子机制研究[J].临床输血与检验,2016,18(5):424-426,427,4.基金项目
本课题受江苏省重点研发计划社会发展项目（No. BE2015717)，江苏省“十二五”期间“科教兴卫工程”医学重点人才（No.RC2011088)，江苏省第四期“333高层次人才培养工程”，江苏省第十批“六大人才高峰”项目（No.2013-WSW-039），2010、2014年度江苏省卫生国际交流支撑计划项目资助 （）
