临床输血与检验2016，Vol.18Issue(5)：427-429,3.DOI:10.3969/j.issn.1671-2587.2016.05.006
1例新等位基因HLA-DRB1*0918的筛查及序列分析
A New Allele Example of Search and Sequence Analysis of HLA-DRB1*0918
摘要
Abstract
Objective To understand and confirm the new human leukocyte antigen (HLA) allele and analyze its nucleotide sequence. Methods Using the direct sequencing method of polymerase chain reaction (PCR–SBT), we analyzed 7 500 hematopoietic stem cell donators with HLA-DRB1 high-resolution classification in Yueyang area and found one example appeared close to HLA-DRB1*09:01:02 sequence of new allele. We made confirmation with the sequencing technique SSP sequencing primers which used for DRB1*09 site specificity and verified the its differences from DRB1*09:01:02 sequence. Results The gene in exon 2 compared with DRB1*09:01:02 276 C>A, its mutations can lead to codon AGC>AGA, 63 S(ser)>R(arginine), has been submitted to GenBank, the gene sequence Numbers for 1523488. Conclusion The allele was obtained by the nucleotide sequence analysis of the new HLA-DRB1 allele, which was named as HLA-DRB1*09:18 in May22, 2013 by the World Health Organization (WHO) HLA Nomenclature Committee.关键词
新等位基因/测序/人类白细胞抗原Key words
New allele/Sequencing/HLA分类
医药卫生引用本文复制引用
刘锋,孙昂,粟玉萍,代敏..1例新等位基因HLA-DRB1*0918的筛查及序列分析[J].临床输血与检验,2016,18(5):427-429,3.
