临床输血与检验2016，Vol.18Issue(5)：434-436,3.DOI:10.3969/j.issn.1671-2587.2016.05.008
1例HLA新等位基因B*15：201*的发现及确认
Discovery and Idenfication a New Allele of HLA-B*15:201
摘要
Abstract
Objective To sequence and identify a novel HLA allele. Methods PCR-SBT method was used for HLA typing and an anomaly allele that is associated with HLA-B locus was analyzed, and sequence-specific sequencing primer(SSSP)was used for sequencing so as to confirm the site of mutation. Results The results showed an new allele of HLA-B locus. SSSP sequencing indicated that the nucleotide sequence of the novel allele is different from the highest homology allele B* 15:01:01: 01 in the second exon at position 662 A>T, resulting in the amino acid sequence at position 221 of B *15:01:01:01, mutated from histidine(H) to leucine(L). Conclusion This is a novel HLA-B allele and has been named HLA-B* 15:201 by WHO HLA Nomenclature Committee.关键词
HLA/新等位基因/分型/测序Key words
HLA/A novel allele/Typing/Sequencing分类
医药卫生引用本文复制引用
王振雷,赵佳,胡光磊,钱明明,李茵,苏蔓,张蓓,李醒,何路军..1例HLA新等位基因B*15：201*的发现及确认[J].临床输血与检验,2016,18(5):434-436,3.基金项目
本课题受2012年度医学适用技术跟踪项目（No.GL2012020）资助 （）
