临床输血与检验2016，Vol.18Issue(5)：448-451,4.DOI:10.3969/j.issn.1671-2587.2016.05.014
某地区献血人群乙型肝炎病毒携带者基因分型与耐药性变异研究
Research for HBV Genotype and Mutation of Drug Resistance in Blood Donation Population in a Given Area
肖泽斌 1林树波 1甘小洪 2郑泽旋 1李娟 2Allen Chan 2温国强1
作者信息
- 1. 515064 广东省汕头市中心血站
- 2. 广州立菲达安诊断产品技术有限公司
- 折叠
摘要
Abstract
Objective To analyze the HBV poisonous genotype and its mutation of drug resistance in blood donation population in a given area. Methods Selected the blood examples from donors since April 1, 2014 to December 31, 2014 and screened out 201 double positive specimens using ELISA double reagent. And then by fluorescence quantitative PCR, 99 cases of HBV positive specimens were screened out. 45 of them held a viral load larger than 1 000 IU/ml specimens, their HBV drug resistance gene were detected (PCR sequencing method). Finally, we analyzed the relationship between the hepatitis b patient virus poisonous genotype and pills resistance segment mutation of this city. Results All 45 cases was Hepatitis B wild-type, no drug resistance mutation was found. Hepatitis B parting with 45 cases of type B was 43 cases, classification rate was 95.6%, the other 2 cases was type C, classification rate was 4.4%. Type B gene classification rate was significantly higher than C gene classification rate, with statistical significance (χ2 =9.256,P<0.05). Conclusion The city's Hepatitis B blood donators hold virus genes model B type at large and the resistance genes are mainly wild type, 45.5% of them has a virus viral load high to 1 000 IU/ml, which need track treatment, providing prevention and cure references for Hepatitis B.关键词
乙型肝炎病毒/基因型/变异/耐药性Key words
Hepatitis b virus/Genotypes/Mutation/Drug resistance分类
医药卫生引用本文复制引用
肖泽斌,林树波,甘小洪,郑泽旋,李娟,Allen Chan,温国强..某地区献血人群乙型肝炎病毒携带者基因分型与耐药性变异研究[J].临床输血与检验,2016,18(5):448-451,4.
