临床小儿外科杂志2016，Vol.15Issue(4)：363-367,5.DOI:10.3969/j.issn.1671-6353.2016.04.016
钙视网膜蛋白在肠无神经节细胞症诊断中的应用
Application of calretinin in the diagnosis of Hirschsprung’s disease
摘要
Abstract
Objetive To analyze retrospectively the clinical significance of calretinin (CR)in the diag-nosis of Hirschsprung’s disease (HD). Methods The expression of calretinin was detected and hematoxylin&eosin (HE)staining performed for spastic and expanding segments of HD and Hirschsprungs disease allied disorder from January 1,2011 to December 31,2014. Results Among them,111 /114 cases had no preoper-ative expression of calretinin in spastic segment of HD.And 112 /114 cases had no ganglion cells.None of 133 cases had an expression of calretinin in spastic segment and there were no ganglion cells.All 133 expanding segments had a positive postoperative expression of calretinin.HE staining showed that all 133 cases had nor-mal ganglion cells.Four cases had no preoperative expression of calretinin in spastic segment of Hirschsprungs disease allied disorder,HE staining showed 3 cases without ganglion cells and 1 case with dysplasic ganglion cells.All 42 cases had an expression of calretinin in involved segments.All 42 cases had dysplasic ganglion cells or fewer ganglion cells. Conclusions Calretinin may be used for aiding the diagnosis of HD.When cal-retinin staining is used for diagnosing HD preoperatively,we should watch out for false positive and false nega-tive results,especially during rectal mucosal biopsy.关键词
钙视网膜蛋白/Hirschsprung 病/免疫组织化学Key words
Calretinin/Hirschsprung Disease/Immunohistochemistry引用本文复制引用
侯金凤,向丽,金先庆,李晓庆..钙视网膜蛋白在肠无神经节细胞症诊断中的应用[J].临床小儿外科杂志,2016,15(4):363-367,5.基金项目
1，国家临床重点专科建设项目，项目号国卫办医函【2013】544；2，重庆市卫生局科研项目（钙视网膜蛋白在婴幼儿先天性无神经节细胞症的早期诊断的临床意义）。 （）
