河南科技大学学报（医学版）2016，Vol.36Issue(3)：161-164,4.DOI:10.15926/j.cnki.issn1672-688x.2016.03.001
人胚肺二倍体细胞衰老程度评价生物学指标研究
Study of Biological Indicators of Cellular Senescence Degree of Human Embryo Lung Diploid Cells
摘要
Abstract
Objective To detect the cell growth state and evaluate the biological indicators of senescence degree of human embryo lung diploid cells by growth curve and flow cytometry assay. Methods The Walvax-2 cells were splited into 16 generations to detect the cell proliferative ability and cell vitality in different generations of Walvax-2 cells by drawing growth curve.The cell cycle phase and cell apoptosis were analyzed by fluorescence activating cell sorter (FACS)assay on different generations Walvax-2 cells with propidium iodide(PI). Results The cell vitality of P20 and P30 were stronger than the P40 and P50 (all P <0.05),and that of P40 was stronger than the P50 (P >0.05).The cell percentage of G0 /G1 phase has no association with the generations of Walvax-2 cells (P >0.05),however the synthesis phase,G2 /M phase and cellular debris were significantly associated with the generations of Walvax-2 cells growth (all P <0.05). Conclusion Drawing growth curve can distinguish cell vitality of Walvax-2 cells which were separated by large generations.The cell cycle changes are detected by fluorescence activating cell sorter assay with propidium iodide and it can act as a biological indicator to evaluate the senescence degree of Walvax-2 cells.关键词
细胞衰老/细胞周期/细胞生长曲线/衰老评价Key words
cellular senescence/cell cycle/cell growth curve/senescence evaluation分类
生物科学引用本文复制引用
聂爱婷,马波,欧俊兴,胡利平,饶窼,聂胜洁..人胚肺二倍体细胞衰老程度评价生物学指标研究[J].河南科技大学学报（医学版）,2016,36(3):161-164,4.基金项目
国家科技部863计划 （）
