河南科技大学学报（医学版）2016，Vol.36Issue(3)：170-173,4.DOI:10.15926/j.cnki.issn1672-688x.2016.03.003
STAT3、Nanog 基因在食管癌组织中的表达及临床病理特征相关性
Expression of STAT3 and Nanog Gene in Esophageal Cancer and Its Correlation with the Clinicopathological Characteristics
陆晓 1冯笑山 1高社干 1周福有 2王能超 2杨海军2
作者信息
- 1. 河南科技大学第一附属医院，河南洛阳 471003
- 2. 安阳市肿瘤医院，河南安阳 455000
- 折叠
摘要
Abstract
Objective To explore STAT3 and Nanog gene expression in esophageal cancer tissues and its correlation with the clinicopathological characteristic in order to look for new targets of gene therapy for esophageal cancer. Methods There were 50 cases of esophageal cancer which was selected from the Fourth Affiliated Hospital of Henan University of Science and Technology in 2014 as the research object.The expression of STAT3 and Nanog mRNA was detected by RT-PCR in esophageal carcinoma and paracancerous tissues.Meanwhile the expression and localization of STAT3 and Nanog protein were detected by Western blot technique and immunohistochemical technique in same tissues. Results The mRNA and protein expression of STAT3 and Nanog in esophageal cancer tissues was significantly higher than that in paracancerous tissue(P <0.05 ).The expression of STAT3 and Nanog in poorly differentiated esophageal cancer tissues was observably higher than that in other differentiated cancer tissues (P <0.05 ).Among esophageal cancer tissues,without lymph node metastasis cancer tissues were accompanied with significantly lower positive expression rates than the tissues with lymph node metastasis(P <0.05).The esophageal cancer tissues without distant metastasis have less expression of STAT3 and Nanog than the tissues with distant metastasis(P <0.05 ).The expression of STAT3 and Nanog in Esophageal cancer tissues showed positive correlation(r =0.303,P <0.05 ). Conclusion STAT3 and Nanog gene may play an important role in carcinogenesis and development of esophageal cancer,and it might be the new target genes for diagnosis and treatment of esophageal cancer.关键词
食管癌/STAT3/Nanog/RT-PCR/Western blot/免疫组化Key words
esophagus cancer/STAT3/Nanog/RT-PCR/Western blot/immunohistochemisty分类
医药卫生引用本文复制引用
陆晓,冯笑山,高社干,周福有,王能超,杨海军..STAT3、Nanog 基因在食管癌组织中的表达及临床病理特征相关性[J].河南科技大学学报（医学版）,2016,36(3):170-173,4.
