林业工程学报2016，Vol.1Issue(5)：52-57,6.DOI:10.13360/j.issn.2096-1359.2016.05.010
黑曲霉固态发酵产阿魏酸酯酶及酶解麸皮制备阿魏酸
Production of feruloyl esterase by solid-state fermentation of Aspergillus niger and preparation of ferulic acid from wheat bran by enzymatic hydrolysis
摘要
Abstract
Ferulic acid is the most abundant hydroxycinnamic acid in plant cell wall, it is mainly linked to hemicellu⁃losic carbohydrate moieties and lignin components. Due to the unsaturation of the aliphatic side chain and its bulk, feru⁃lic acid can form stable phenoxy motieties and serve as good antioxidants. Ferulic acid is also a precursor to vanillin and other high⁃valued aromatic compounds synthesis. Ferulic acid has large number of applications in health, food, pharma⁃ceutical, and cosmetics industries. De⁃strached wheat bran contains 5 mg/g to 10 mg/g of ferulic acid and is considered as a potential resource for ferulic acid production. In this study, feruloyl esterase ( FAE) was prepared by solid⁃state fer⁃mentation of Aspergillus niger TRIIM 3�00954 derived from 10 different strains. The results showed that the maximum en⁃zyme activity was produced under the conditions as:the ratio of wheat bran to bagasse at 2∶1, the 0�6% glucose as sup⁃plementary carbon source, the 1% urea as supplementary nitrogen source, the initial pH of 6�0, and culture temperature at 30 ℃. After 7 d of fermentation, 8�2 U/g of feruloyl esterase was obtained. The optimum pH value and temperature of the FAE were 5�0 and 50℃, respectively. The optimum enzymatic hydrolysis condition is obtained as follows:tempera⁃ture 50 ℃ and pH 6�5. Under this condition, the maximum of 60�6% total ferulic acid was released from de⁃starched wheat bran after 6 h enzymatic hydrolysis using 0�6 U/g and feruloyl esterase and 90 U/g xylanase. The economic and eco⁃friendly ferulic acid recovery of phenolic acids from the agricultural and forestry by⁃products to produce value⁃added products has become a research focus in recent years. This paper provides an effective technological approach to extract ferulic acid from de⁃starched wheat bran.关键词
黑曲霉/阿魏酸/阿魏酸酯酶/固态发酵/麸皮Key words
Aspergillus niger/ferulic acid/ferulic acid esterase/solid state fermentation/wheat bran分类
生物科学引用本文复制引用
赵浩源,张迎亚,蒋侃侃,龙良鲲,丁少军..黑曲霉固态发酵产阿魏酸酯酶及酶解麸皮制备阿魏酸[J].林业工程学报,2016,1(5):52-57,6.基金项目
国家林业局“948”项目（2013－4－16）。 （）
