林业科学2016，Vol.52Issue(8)：38-45,8.DOI:10.11707/j.1001-7488.20160805
辽宁杨雄花序发育及花粉萌发特征
Characteristics of Staminate Catkin Development and Pollen Germination in Populus × liaoningensis
摘要
Abstract
[Objective]In order to develop genetically improved liaoning poplar ( Populus × liaoningensis) ,the process of flowering and pollen germination were observed and analyzed.[Method]The process of flowering,pollen germination in vitro and the effect of different storage methods on pollen germination and growth of pollen tubes were investigated through water culture,pollen germination in vitro and pllen storing by means of drawing model pattern and graphic interpretation to systematically study the development of staminate catkin and pollen dispersal,and pollen germination in vitro,and to explore the impacts of different storage conditions on pollen germination and growth of pollen tubes.[Result]1) Four stages such as bud time,extension period and so on belong to the course of the flowering. It will spent 12 days. After the anthers mature, it was easy to be dispersed for the pollen with slightly vibrate. 2) The optimal culture medium for germination was 210 g·L -1 sucrose + 30 mg·L -1 H3 BO3 + 10 mg·L -1 CaCl2 ,the rate of germination was up to 58. 57%. Effects of temperature, sucrose,H3BO3 and CaCl2were remarkable in orthogonal design experiment(P≤0. 05). The best culture medium for growth of pollen tubes was 150 g·L -1 sucrose + 40 mg·L -1 H3 BO3 + 10 mg·L -1 CaCl2 ,the average length of tubes was 753. 20 μm in seventh group. 3) The pollen tubes have no longer extended after 7 h culture in dark. 4) The germination rate was higher than any other temperatures(4 ℃ and room temperature) cultivating under the temperature of -20 ℃ and it ’s similar to the pollen tubes. When we laid aside the pollen in a constant temperature,as the time went on,the germination got lower and the length of the pollen tubes got shorter.[Conclusion]The pollen should be collected in the third stage to avoid the pollen loss. The relationship between pollen tubes and germination rates was no significant by statistic analysis. Pollen stored in a lower temperature had a higher viability,meanwhile,it took a shorter time for pollen tubes to reach to a same length. Compared with the fresh pollen,if the stored pollen was used to cross experiment,the times of the suitable pollination should be increased and the interval between the two fertilization should be extended.关键词
辽宁杨/花粉/离体萌发/贮藏条件Key words
Populus × liaoningensis/pollen/in vitro germination/storage分类
农业科技引用本文复制引用
许东,李际红,谢兰禹,亓晓,王锦楠,邢世岩..辽宁杨雄花序发育及花粉萌发特征[J].林业科学,2016,52(8):38-45,8.基金项目
山东省农业良种工程项目“优质用材树种和抗逆生态防护树种良种选育”(2013-2016) （2013-2016）
山东省自然基金“窄冠黑白杨杂种优势遗传机理的研究”(ZR2013CM020)(2014-2016)。 （ZR2013CM020）
