林业科学2016，Vol.52Issue(8)：146-156,11.DOI:10.11707/j.1001-7488.20160818
衡阳紫色土丘陵坡地不同植被恢复阶段土壤微生物群落多样性的变化
Variations of Soil Microbial Community Diversity in Purple Soils at Different Re-Vegetation Stages on Sloping-Land in Hengyang, Hunan Province
摘要
Abstract
[Objective]The aim of this study was to explore variation characteristics of soil microbial community diversity in purple soils at different re-vegetation stages on sloping-land in Hengyang,Hunan Province,South-central China.[Method]By using the spatial series to replace time series,four typical vegetation types,bare land,herbaceous and shrubby communities,as well as arboreal community,were selected to study the soil microbial community diversity by using the Biolog-ECO micro-plate technique. The four communities in purple soils on sloping-land were similar,and typical,and denoted four different re-vegetation stages.[Result]The soil microbial metabolic activity increased after re-vegetation(P < 0.05) significantly; The average well color development(AWCD),which is used to represent soil microbial activity and functional diversity,followed the order of arboreal community > shrubby community > herbaceous community >bare land at the different re-vegetation stages,and followed the order of 0 ~10 cm soil layer>10 ~25 cm soil layer>25 ~40 cm soil layer in the different soil layers at the same re-vegetation stage; The Shannon-Wiener species richness index( H) ,Shannon-Wiener evenness Index( E) ,Simpson dominance index( D) and McIntosh index( U) at four different re-vegetation stages were the highest in arboreal community(P<0. 05),the second in herbaceous community and shrubby community( P >0. 05 ) ,and the lowest in bare land ( P <0. 05 ); The carbon and nitrogen source utilization intensities followed the order of arboreal community>shrubby community>herbaceous community>bare land at different re-vegetation stages(P<0. 05),and followed the order of 0 -10 cm soil layer>10 -25 cm soil layer>25 -40 cm soil layer in different soil layers(P<0. 05); Principal component analysis(PCA) showed that two factors in relation to carbon and nitrogen sources from 31 carbon and nitrogen types could explain 37. 24% and 15. 89% of the variation,and carbohydrates,amino acids,intermediate metabolites,and secondary metabolites were the main carbon and nitrogen sources separating the two principal component factors; The results of correlation analysis indicated that total organic carbon( TOC) ,total nitrogen( TN) ,total phosphorus( TP) and available phosphorus( AP) had important influence on soil microbial community diversity. [Conclusion]All the results indicated that re-vegetation could enhance the soil microbial metabolic function which was beneficial to the reproduction of soil micro-organisms,thereby promoting an increase in soil carbon and nitrogen source utilization intensities.关键词
Biolog-ECO/植被恢复/土壤微生物群落/多样性/紫色土/衡阳Key words
Biolog-ECO/re-vegetation/soil microbial community/diversity/purple soils/Hengyang分类
农业科技引用本文复制引用
邹冬生,杨满元,付美云,林仲桂,赵林峰..衡阳紫色土丘陵坡地不同植被恢复阶段土壤微生物群落多样性的变化[J].林业科学,2016,52(8):146-156,11.基金项目
湖南省科技厅项目(S2006N332) （S2006N332）
湖南省教育厅科学研究项目(12C1057) （12C1057）
湖南省林业科技创新计划项目(XLK201341) （XLK201341）
湖南省衡阳市农业科技支撑计划项目(2014KN27) （2014KN27）
湖南省普通高校优秀青年骨干教师培养对象资助项目和湖南环境生物职业技术学院南岳学者基金项目。 （）
