药学研究2016，Vol.35Issue(9)：501-507,7.DOI:10.13506/j.cnki.jpr.2016.09.002
阿胶加工工艺过程中DNA降解规律研究
DNA degradation of Colla Corii Asini during processing
摘要
Abstract
Objective In this paper,each key processing stage samples of Colla Corii Asini as the research object to explore for DNA degradation of process variation in Colla Corii Asini. Methods Extractive DNA was evaluated by ultra trace spectrophotometric meter detection,agarose gel electrophoresis,PCR,fluorogenic quantitative PCR and SSR microsat-ellite CE detection.Results The results showed that in raw materials,melting stage,dual processing stage,before the con-densed phase and adding accessories stage can detect clear amplified bands in the size range in the 200~1 600 bp fragment. The degradation of DNA was the most serious in the sample of the gel paste,and the range of 100~800 bp could only be de-tected.Conclusion With the processing of Colla Corii Asini product,DNA quality was gradually declined,and that DNA degraded most seriously at the glue paste stage,but the genomic DNA was still competent for Colla Corii Asini materials i-dentification and DNA traceability.关键词
驴皮/阿胶/DNA降解/质量检测/分子生物学Key words
Donkey hide/Colla Corii Asini/DNA degradation/Quality detection/Molecular biological分类
生物科学引用本文复制引用
刘艳艳,董书光,耿加亮,谭晴晴,张全芳,步迅..阿胶加工工艺过程中DNA降解规律研究[J].药学研究,2016,35(9):501-507,7.基金项目
济南市高校院所自主创新项目 （）
