药学研究2016，Vol.35Issue(9)：523-525,3.DOI:10.13506/j.cnki.jpr.2016.09.008
HPLC法测定咽喉清口含片中哈巴俄苷及肉桂酸的含量
Determination of harpagoside and cinnamic acid in Yanhouqing Buccal Tablets by HPLC
摘要
Abstract
Objective To establish a method for the determination of harpagoside and cinnamic acid in Yanhouqing Buccal Tablets.Methods Separations were carried out on a Waters Symmetry C18 column(4.6 mm×250 mm,5μm)by gra-dient elution with 0. 3% acetic acid and acetonitrile,the detection wavelength was 279 nm,the flow rate was 1. 0 mL·min-1 ,and the column temperature was 30 ℃. Results Chromatographic peaks of harpagoside and cinnamic acid were well separated with good precision and stability.Then the standard curve of harpagoside was rectilinear within the range of 0.039 65~0.396 5 μg(r=0.999 8),and the average recovery was 98.13 % with RSD of 1.20 %(n=6).The standard curve of cinnamic acid was rectilinear within the range of 0.099 68~0.996 8μg( r=0.999 7),and the average recovery was 98.87 % with RSD of 1.17%(n=6).Conclusion The method was accurate and reproducible,and can be used for the de-termination of cinnamic acid and harpagoside in Yanhouqing Buccal Tablets.关键词
咽喉清口含片/哈巴俄苷/肉桂酸/含量测定Key words
Yanhouqing Buccal Tablets/Harpagoside/Cinnamic acid/Determination分类
医药卫生引用本文复制引用
李一圣,李文周,卫平,邱书奇..HPLC法测定咽喉清口含片中哈巴俄苷及肉桂酸的含量[J].药学研究,2016,35(9):523-525,3.基金项目
深圳市重点实验室项目（No.ZDSYS201506050935272）；深圳市龙岗区科技计划项目 （）
