山东医药2016，Vol.56Issue(33)：8-10,3.DOI:10.3969/j.issn.1002-266X.2016.33.003
FrzA基因转导干预对大鼠体外缺血缺氧心肌细胞Wnt信号通路相关分子表达及细胞凋亡的影响
Effects of FrzA gene transduction on Wnt signaling pathway expression and apoptosis of cardiomyocytes in rats with ischemia-hypoxia in vitro
摘要
Abstract
Objective To investigate the effects of recombinant adeno-associated virus type 9 (rAAV9)carrying FrzA gene transduction in inhibiting Wnt signaling pathway activity and on myocardial apoptosis of cardiomyocytes in rat models with ischemia-hypoxia.Methods The myocardial cells of SD rats were isolated and cultured.A recombinant AAV9 vector carrying FrzA gene was transfected into cardiomyocytes and then a hypoxia model was created.We divided them into four groups:control group,ischemia-hypoxia group,ischemia-hypoxia +empty virus (rAAV9-CMV-GFP)group (empty virus group)and ischemia-hypoxia +rAAV9-CMV-FrzA group (FrzA group).RT-PCR was used to detect the expression of tar-get gene FrzA,and Western blotting was applied to detect the levels of Dvl-1,β-catenin and c-Myc as well as expression of apoptosis-related proteins Bcl-2 and Bax.Results On the fifth day after virus transfection,the FrzA target gene was highly expressed in cardiomyocytes (all P <0.05).The expression of Wnt signaling pathway molecules Dvl-1,β-catenin and c-Myc was significantly higher in the ischemia-hypoxia group,empty virus group and FrzA group,and the ratio of Bax/Bcl-2 was also higher than that of the control group (all P <0.05).The expression of Wnt signaling pathway molecules Dvl-1,β-catenin and c-Myc was significantly lower in FrzA group,and the ratio of Bax/Bcl-2 was also lower than that of the ische-mia-hypoxia group and empty virus group (all P <0.05).Conclusion rAAV9 carrying FrzA gene can effectively inter-vene the Wnt signaling pathway and inhibit its activity and thus reduce the apoptosis of cardiomyocytes.关键词
心肌细胞/缺血缺氧/重组9型腺相关病毒/FrzA基因/Wnt信号通路/细胞凋亡Key words
cardiomyocytes/ischemia-hypoxia/recombinant adeno-associated virus type 9/FrzA gene/Wnt signa-ling pathway/apoptosis分类
医药卫生引用本文复制引用
李华垠,陶静,马依彤,刘芬,陈邦党..FrzA基因转导干预对大鼠体外缺血缺氧心肌细胞Wnt信号通路相关分子表达及细胞凋亡的影响[J].山东医药,2016,56(33):8-10,3.基金项目
国家自然科学基金资助项目（81470468）；新疆维吾尔自治区研究生创新基金资助项目（XJGRI2015064）。 （）
