山东医药2016，Vol.56Issue(33)：22-24,3.DOI:10.3969/j.issn.1002-266X.2016.33.007
siRNA抑制X连锁凋亡抑制蛋白基因对人HeLa细胞凋亡及周期的影响
XIAP gene inhibited by siRNA and its effect on apoptosis and cell cycle of HeLa cells
摘要
Abstract
Objective To explore the inhibitory effect of small interfering RNA(siRNA)silencing human X-linked inhibitor of apoptosis protein (XIAP)gene on cell cycle and apoptosis of cervical HeLa cells.Methods DNA template coding XIAP specific siRNA was designed and synthesized.The Hela cells were divided into 4 groups:the observation group (pGPU6 /GFP/Neo /XIAP-siRNA-transfected cells),blank group (not added with siRNA and transfection reagent), negative control group (NC group,transfected by disrupted siRNA)and transfection reagent control group (Mock group, added with transfection reagents and without siRNA).The XIAP-siRNA was transfected into HeLa cells.The expression level of XIAP mRNA was assayed by RT-PCR.The apoptosis rate and cell cycle distribution were analyzed by flow cytome-try.Results Significant difference was found in the expression of XIAP mRNA at 48 and 72 h after transfection between the observation group and blank group (all P <0.05),but no significant difference was found among the blank group, Mock group and NC group (all P >0.05).In the observation group,the interference rates of HeLa cells transfected with XIAP-siRNA at 48 and 72 h were 27% and 59%.The apoptotic rate of the observation group was higher than that of the blank group (all P <0.05).Significant difference was found in the apoptosis rate of the observation group between the transfection of 48 h and 72 h (P <0.05).Compared with the blank group,the cells in the G0-G1 phrase increased and then decreased in the S phrase at 48 and 72 h after transfection (all P <0.05).Condnsion Specific XIAP-siRNA can ef-fectively silence the XIAP gene of cervical cancer Hela cells,inhibit the proliferation and promote the apoptosis.关键词
宫颈肿瘤/Hela细胞/X连锁凋亡抑制蛋白/微小干扰iRNAKey words
cervical neoplasms/Hela cells/X-linked inhibitor of apoptosis protein/small interfering RNA分类
医药卫生引用本文复制引用
唐薇薇,郑洪,厉国慧,王琳琳..siRNA抑制X连锁凋亡抑制蛋白基因对人HeLa细胞凋亡及周期的影响[J].山东医药,2016,56(33):22-24,3.基金项目
贵州省科技厅基金资助项目（黔科合 J 字［2007］2128号）。 （）
