山东医药2016，Vol.56Issue(34)：1-3,3.DOI:10.3969/j.issn.1002-266X.2016.34.001
姜黄素对 TNF-α诱导人结直肠癌细胞上皮间充质转化、迁移的影响
Effect of curcumin on epithelial-mesenchymal transition and migration of colorectal cancer cells induced by TNF-α
摘要
Abstract
Objective To investigate the effect of curcumin on tumor necrosis factor-α(TNF-α)-induced epithelial-mesenchymal transition (EMT)of colorectal cancer HCT116 cells and the possible mechanism.Methods The human color-ectal cancer HCT116 cells were divided into three groups.Group A and group B were treated with 20 ng/mL TNF-α,20 ng/mL TNF-α +25 μmol/L curcumin for 48 h,respectively,and group C with routine culture.Using phase contrast microscope to observe the changes in cell morphology,cell scratch experiments to observed cell migration in each group.Western blotting was used to detect the expression levels of EMT markers E-cadherin,Vimentin and NF-κB signaling pathway-related P65 pro-tein.Results Cells of group A were mostly spindle-shaped,fusiform and loosely arranged and most appeared elongated pseudopodia;in the group B,the slender pseudopodia cells were less,and apoptotic cells were more than those in the group A;most of cells in the group C were polygon,there was a little space between cells,and a few cells appeared stubby pseud-opodia.The cell migration distance of groups A,B and C were (319.84 ±20.93)μm,(90.70 ±7.25)μm,(144.07 ± 11.31)μm,respectively,and significant difference was found between these three groups (all P <0.05).Compared with group C,the expression of E-cadherin in cells of group A was decreased,however,the expression of Vimentin and p65 pro-tein was increased;and compared with group A,the expression of E-cadherin in cells of group B was increased,the expres-sion of Vimentin and p65 protein was reduced.Conclusion Curcumin inhibited the occurrence,development and metastasis of TNF-α-induced EMT in HCT116 colorectal cancer cells probably through NF-κB signaling pathway.关键词
结直肠癌/HCT116细胞/姜黄素/上皮间质转化/核转录因子κB/肿瘤坏死因子Key words
colorectal carcinoma/HCT116 cells/curcumin/epithelial-mesenchymal transition/nuclear transcription factor-κB/tumor necrosis factor引用本文复制引用
李倩,徐春燕,彭鹏,覃蒙斌,李素艳,黄杰安..姜黄素对 TNF-α诱导人结直肠癌细胞上皮间充质转化、迁移的影响[J].山东医药,2016,56(34):1-3,3.基金项目
国家自然科学基金资助项目（81260365）；广西中医药民族医药自筹经费科研课题项目（GZZC14-57）。 （）
