山东医药2016，Vol.56Issue(34)：8-10,3.DOI:10.3969/j.issn.1002-266X.2016.34.003
川芎素对结直肠癌Lovo细胞生长的体内外抑制作用及机制
Inhibitory effect of sodium ferlate on proliferation of human colon cancer Lovo cells in vitro and in vivo
摘要
Abstract
Objective To investigate in vitro and in vivo inhibitory effects of sodium ferlate on the proliferation of hu-man colon cancer Lovo cells as well as the relevant mechanism.Methods Lovo cells were divided into two groups:the observation group which was co-cultured with sodium ferlate with the final concentrations of 0.125,0.25,0.5 and 1.0 mg/mL,and the control group without any sodium ferlate.The optical density of Lovo cells was determined by MTT.The vitali-ty index was calculated.The effects of sodium ferlate on the cell cycle and apoptosis of Lovo cells were analyzed by flow cy-tometry.Fourteen nude mice bearing Lovo cells were divided into two groups:the observation group which was injected with 50 μL sodium ferlate with the concentration of 0.5 mg/mL,and the control group which was injected by the same amount of normal saline,every other day,for 7 times and the tumor volume was measured before and after medication.The expres-sion of Bcl-2 and pro-Caspase-3 in Lovo cells and tumor tissues was determined by Western blotting.Results The cell vitality indexes were respectively 81.23 ±6.58,75.69 ±6.34,62.18 ±3.29 and 58.39 ±2.69 in the observation group after 24-hour treatment of sodium ferlate with the final concentrations of 0.125,0.25,0.5 and 1.0 mg/mL.The cell pro-portion ratio of G1 phase was decreased,and was decreased in the S phase (all P <0.05),meanwhile,the cell apoptosis rate was increased (P <0.05).Compared with the control group,the tumor volume decreased in the tumor-bearing nude mice of the observation group (all P <0.05);and the expression of Bcl-2 and pro-Caspase-3 was decreased in the Lovo cells and tumor tissues of nude mice in the observation group (all P <0.05).Conclusion Sodium ferlate may inhibit the expression of Bcl-2,activate caspase-3 and promote the apoptosis,and thus inhibit the proliferation of Lovo cells.关键词
结直肠癌/川芎素/Lovo细胞/细胞凋亡/B淋巴细胞瘤-2基因/半胱氨酸的天冬氨酸蛋白水解酶Key words
colon carcinoma/sodium ferlate/Lovo cells/apoptosis/B-cell lymphoma-2 gene/Caspase-3分类
医药卫生引用本文复制引用
金剑,赵庆..川芎素对结直肠癌Lovo细胞生长的体内外抑制作用及机制[J].山东医药,2016,56(34):8-10,3.基金项目
上海市卫生和计划生育委员会科研课题（20144Y01116）。 （）
