山东医药2016，Vol.56Issue(35)：9-12,4.DOI:10.3969/j.issn.1002-266X.2016.35.003
转染人源 Omentin-1、Vaspin 妊娠期糖尿病脂肪细胞胰岛素受体底物和磷脂酰肌醇3激酶表达变化
Expression changes of IRS-1/2 and PI3K in GDM adipocyte cells after anthropogenic Omentin-1, Vaspin transfection
摘要
Abstract
Objective To observe the expression changes of insulin receptor substrate-1/2 ( IRS-1/2) and phosphati-dy inositol 3 kinase (PI3K) in gestational diabetes mellitus (GDM) adipocyte cells after anthropogenic Omentin-1, visceral adipose tissue-derived serine protease inhibitor ( Vaspin) transfection.Methods Recoveried, extended and differentiated GDM preadipocyte cells were prepared, and then we built Omentin-1, Vaspin overexpression carrier, and transfected the extended adipose cells with different overexpression levels (1.0, 2.5 and 5.0μg).Meanwhile, no transfection group was taken as the contrast.Using Q-PCR to detect the mRNA expression of Omentin-1, Vaspin, IRS-1/2 and PI3K (P85a), using Western blotting to detect protein expression of Omentin-1, Vaspin, IRS-1/2 and PI3K ( P85a) as well as IRS-1/2 phosphorylation levels, using [3H]-2-deoxidation-D-glucose uptake assay to detect the rates of glucose uptake in different transfection groups.Results With the increasing Omentin-1 expression, the mRNA and protein expression of IRS-1 and PI3K(P85a) were increased, but those of IRS-2 had no significant changes, IRS-1 phosphorylation increased obviously, IRS-2 phosphorylation had no significant changes, and the glucose uptake rate increased significantly.With the increasing Vaspin expression, all of the mRNA, protein and phosphorylation in IRS-1/2 and PI3K(P85a) had no significant changes, so did the glucose uptake rates.Conclusion In GDM adipocyte cells after anthropogenic Omentin-1 transfection, IRS-1, PI3K( P85a) and glucose uptake rates were increased, but no changes in those of GDM adipocyte cells after anthropogenic Vaspin transfection.关键词
妊娠期糖尿病/网膜素1/内脏脂肪组织源性丝氨酸蛋白酶抑制剂/基因转染/胰岛素抵抗/胰岛素受体底物1/胰岛素受体底物2/磷脂酰肌醇3激酶Key words
gestational diabetes mellitus/Omentin-1/visceral adipose tissue-derived serine protease inhibitor/gene transfection/insulin resistance/insulin receptor substrate-1/insulin receptor substrate-2/phosphatidy inositol 3 kinase分类
医药卫生引用本文复制引用
潘宝龙,马润玫..转染人源 Omentin-1、Vaspin 妊娠期糖尿病脂肪细胞胰岛素受体底物和磷脂酰肌醇3激酶表达变化[J].山东医药,2016,56(35):9-12,4.基金项目
国家自然科学基金资助项目（81160082）。 （）
