山东医药2016，Vol.56Issue(35)：13-16,4.DOI:10.3969/j.issn.1002-266X.2016.35.004
转染 miR-101类似物后卵巢癌细胞对紫杉醇敏感性的变化及其机制
Sensitivity change of ovarian cancer cells transfected with miR-101 analogues to paclitaxel
摘要
Abstract
Objective To observe the sensitivity change of ovarian cancer cells transfected with miR-101 analogues to paclitaxel, and to explore its mechanism.Methods Ovarian cancer cells SKOV3 were cultivated and divided into 3 groups:the observation group, control group and single drug group.Three groups were treated with 0, 6.25, 25, 100, 400 and 1 600 ng/mL paclitaxel.The observation group was transfected with miR-101 mimics, control group was transfected with unrelated sequence and single drug group was not transfected with any sequence.MTT was conducted to observe the proliferation ability of cells in each group.Annexin V/propidium iodide staining was employed to detect the apoptosis rate in each group.Western blotting was employed to detect the protein expression of DNMT3A in these three groups which were respectively treated by 25 ng/mL paclitaxel.Results The OD values of the observation group were 0.632 ±0.014, 0.554 ±0.012, 0.503 ±0.015, 0.428 ±0.011, 0.346 ±0.006 and 0.301 ±0.007, respectively.The OD values of the single drug group were 0.718 ±0.013, 0.687 ±0.015, 0.619 ±0.011, 0.554 ±0.009, 0.473 ±0.008 and 0.420 ±0.011, respectively.The OD values of the control group were 0.713 ±0.016, 0.671 ±0.011, 0.626 ±0.010, 0.549 ±0.008, 0.467 ±0.010 and 0.412 ±0.008, respectively.The differences were of statistical significance between the observation group and control group (all P<0.05).The apoptosis rates of the observation group were 9.63%±0.38%, 14.71%± 0.64%, 21.27%±0.79%, 27.05%±0.92%, 31.82%±1.07%, 35.51%±1.63%, respectively.The apoptosis rates of the single drug group were 3.61%±0.24%, 6.18%±0.48%, 9.57%±0.42%, 15.64%±0.75%, 18.79%±1.12%and 22.05%±1.34%, respectively.The apoptosis rates of the control group was 3.74%±0.29%, 6.02%± 0.35%, 10.63%±0.58%, 15.21 %±0.84%, 19.37%±1.26% and 23.30%±1.18%, respectively.The differ-ences were of statistical significance between the observation group and control group (all P<0.05).The expression of DNMT3A protein in the observation group, control group and single drug group were 0.427 ±0.068, 0.961 ±0.107 and 0.947 ±0.113, respectively.The differences were of statistical significance among these three groups (P<0.05).Con-clusion MiR-101 analog can increase the sensitivity of ovarian cancer cells to paclitaxel through targeted-regulating DN-MT3A.关键词
微小RNA-101类似物/卵巢癌细胞/甲基化转移酶3A/紫杉醇/药物敏感性Key words
miR-101 analogues/ovarian carcinoma cells/methylation transferase 3A/paclitaxel/drug susceptibility分类
医药卫生引用本文复制引用
胡可可,刘小敏,宋泓,于渊毅..转染 miR-101类似物后卵巢癌细胞对紫杉醇敏感性的变化及其机制[J].山东医药,2016,56(35):13-16,4.基金项目
国家自然科学基金资助项目（81101988）。 （）
