山东医药2016，Vol.56Issue(35)：21-23,3.DOI:10.3969/j.issn.1002-266X.2016.35.006
莪术醇与奥沙利铂联合肿瘤内注射对裸鼠胃癌移植瘤生长、瘤细胞增殖的影响及其机制
Effects of curcumol combined with oxaliplatin on xenograft growth and proliferation of gastric cancer tumor cells in nude mice
摘要
Abstract
Objective To observe the effects of curcumol combined with oxaliplatin on xenograft growth of gastric cancer cells in nude mice, then to explore the mechanism.Methods Twenty nude mice were selected to establish the xen-ograft models of gastric cancer cells in nude mice.Nude-mice xenograft models were randomly divided into four groups ( n=5 in each group): the control group, curcumol group, oxaliplatin group and curcumol combined with oxaliplatin group (combined treatment group), then they were respectively treated with normal saline (0.05 mL/10 g), curcumol (100 mg/kg), oxaliplatin (30 mg/kg) and curcumol (100 mg/kg) combined with oxaliplatin (30 mg/kg), once every 4 days for four times.The growth of tumor was recorded.The cell survival rate of tumor cells in cancer tissues was determined by MTT.The expression levels of Caspase-3, Bcl-2 mRNA and protein were detected by fluorescence quantitative RT-PCR and immunohistochemistry.Results Compared with the control group, the tumor volume, weight, cell survival rate and the expression of Bcl-2 was decreased but the expression of Caspase-3 was increased in the curcumol group, oxaliplatin group and combined treatment group, and these changes were more significant in the combined treatment group (all P<0.05). Conclusion The curcumol combined with oxaliplatin may inhibit the xenograft growth and proliferation of gastric cancer tumor cells in nude mice, and the mechanism may be related to the up-regulation of Caspase-3 and down-regulation of Bcl-2.关键词
莪术醇/奥沙利铂/胃癌/裸鼠移植瘤模型/B淋巴细胞瘤2/含半胱氨酸的天冬氨酸蛋白水解酶Key words
curcumol/oxaliplatin/gastric carcinoma/xenograft models in nude mice/Bcl-2, Caspase-3分类
医药卫生引用本文复制引用
王冬,李勇,郭志朋,赵群,范立侨,檀碧波,崔平..莪术醇与奥沙利铂联合肿瘤内注射对裸鼠胃癌移植瘤生长、瘤细胞增殖的影响及其机制[J].山东医药,2016,56(35):21-23,3.基金项目
国家自然基金面上项目（81372580）；河北省中医药管理局科研基金资助项目（2014145D）。 （）
