山东医药2016，Vol.56Issue(35)：24-27,4.DOI:10.3969/j.issn.1002-266X.2016.35.007
转染 ClC-3 siRNA 的大鼠成骨细胞增殖、分化观察
Observation of cell proliferation and differentiation in rat osteoblasts tranfected by ClC-3 siRNA
李琴 1何伶俐 1陈婷婷 1龙密密 1王诚1
作者信息
- 1. 六盘水市人民医院，贵州六盘水553001
- 折叠
摘要
Abstract
Objective To observe the cell proliferation and differentiation in rat osteoblasts which were transfected by ClC-3 siRNA.Methods The primary osteoblasts were extracted and divided into 4 groups:the control group which was not treated, negative control ( NC) group which was transfected by unordered siRNA, Lipo group which was transfected by li-pofectamineTM2000 transfection reagents only and the ClC-3 siRNA group which was transfected by ClC-3 siRNA.All cells were cultured in the flow shear stress installation.The optical density (OD) values at 24, 48 and 72 h after culture in each group were detected by MTT.The alkaline phosphatase level and calcified nodule was also detected.Results Compared with the control group, the OD value of the ClC-3 siRNA group was decreased at each time points (all P<0.05).The al-kaline phosphatase levels and calcified nodule forming abilities in the ClC-3 siRNA, Lipo, NC and control groups were (32.434 ±2.130), (46.199 ±1.900), (47.347 ±2.500) and (49.540 ±1.550) U/mL, and 25.02%±3.46%, 36.83%±4.54%, 38.45%±4.84%and 40.57%±2.22%, respectively.Significant difference was found between the ClC-3 siRNA group and the control group (all P<0.01).Conclusion After transfection of ClC-3 siRNA, the cell prolif-eration and differentiation of rat osteoblasts is inhibited.关键词
基因转染/ClC-3蛋白/成骨细胞/氯通道/流体剪切力/细胞增殖/细胞分化/碱性磷酸酶Key words
gene transfection/ClC-3 protein/osteoblasts/chloride channel/flow shear stress/cell proliferation/cell differentiation/alkaline phosphatase分类
医药卫生引用本文复制引用
李琴,何伶俐,陈婷婷,龙密密,王诚..转染 ClC-3 siRNA 的大鼠成骨细胞增殖、分化观察[J].山东医药,2016,56(35):24-27,4.
