摘要
Abstract
Objective:To establish a simultaneous determination aesculin ,aesculetin ,fraxin ,fraxetin of HPLC method ,provide a quantitative method for quality evaluation of fraxini cortex .Methods:Chromatographic column :Beta‐sil‐C18 (250 mm × 4 .6 mm ,5μm ,the LOT :13697) ,mobile phase:acetonitrile‐0 .1% phosphoric acid aqueous solu‐tion (13∶87) ,detection wavelength:334 nm ,column temperature:30℃ ,the flow rate:1ml/min .Results:Aesculin , aesculetin ,fraxin ,fraxetin in 0 .3090~3 .0900μg ,0 .1275~1 .2750μg ,0 .0276~0 .2760μg ,0 .0540~0 .5400μg (r=0 . 999) have a good linear relationship .Conclusion:This method is simple ,rapid ,good reproducibility ,high accuracy , good chromatographic peak separation .关键词
色谱法,高压液相/秦皮Key words
Chromatography/high pressure liquid Fraxini cortex分类
医药卫生
