天津中医药2016，Vol.33Issue(9)：549-554,6.DOI:10.11656/j.issn.1672-1519.2016.09.11
生理及病理状态下大鼠海马θ节律振荡与空间学习记忆的相关性研究
The correlatation study between hippocampal cholinergic θ rhythm and spatial memory in rats under physiological and pathological conditions
刘旭 1岳兴华 1郑燕萍1
作者信息
- 1. 天津中医药大学第一附属医院临床实验室，天津 300193
- 折叠
摘要
Abstract
[Objective] To investigate the correlation between hippocampal cholinergic θ rhythm and spatial learning and memory under physiological and pathological conditions, further clarify the association between the cognitive deficits and the hippocampal θsynchronization disorder. This article also wants to provide the experimental evidence evaluating the ability of spatial learning and memory in rodents by recording the hippocampal cholinergic θ rhythm. [Methods] Tail-pinch induced hippocampal cholinergic θ rhythm was recorded after Morris water maze test on the same SD rats. Main indicators including: the latencies in the hidden platform test, the percentage of swimming time in target quadrant in the probe trial test, the swimming speed and the latencies in the visible platform test, the peak power (Ppeak) and average peak frequency (Fpeak) of θ rhythm. [Results] Bilateral intrahippocampal injection of full length amyloidβ peptide (Aβ1-42) significantly impaired spatial learning (P<0.05) and memory (P<0.05) of rats in Morris water maze test and reduced the Ppeak of hippocampal cholinergic θ rhythm (P<0.05). In control group, there was a significant negative correlation between the Ppeak of hippocampal cholinergic θ rhythm and the escape latency (r=-0.803,P<0.05) and a significant positive correlation between the Ppeak of hippocampal cholinergic θ rhythm and the percentage of swimming time in target quadrant (r=0.762,P<0.05). Similar correlations were found with a correlation coefficient of r=-0.764 (P<0.05) and r=0.771 (P<0.05) respectively in Aβ1-42 injection group. Taken these as a whole,the Ppeak of hippocampal cholinergic θ rhythm was more correlated with escape latency (r=-0.896, P<0.01) and swimming time percentage (r=0.888, P<0.01). [Conclusions] There is strong correlation between the hippocampal cholinergic θ rhythm and the spatial learning and memory ability in rats, suggesting the somatosensory stimulation induced hippocampal cholinergic θ rhythm may be involved in the spatial learning and memory consolidation in rats. The power of hippocampal cholinergic θ rhythm may be an indicator to assess the ability of spatial learning and memory in rats.关键词
海马θ节律振荡/空间学习记忆/淀粉样 β 蛋白/阿尔茨海默病Key words
hippocampal θ rhythm/spatial learning and memory/amyloid β protein/Alzheimer’s disease分类
医药卫生引用本文复制引用
刘旭,岳兴华,郑燕萍..生理及病理状态下大鼠海马θ节律振荡与空间学习记忆的相关性研究[J].天津中医药,2016,33(9):549-554,6.
