天津中医药2016，Vol.33Issue(9)：559-564,6.DOI:10.11656/j.issn.1672-1519.2016.09.13
血府逐瘀汤不同剂型数字化指纹图谱的构建
The establishment of UPLC-Digitized fingerprinting of multiple dosage forms of Xuefu Zhuyu decoction
摘要
Abstract
[Objective] To establish a sensitive and specific UPLC-digitized fingerprinting of multiple dosage forms of Xuefu Zhuyu decoction (capsule, oral liquid, tablet, soft capsule and Decoction) with UPLC-DAD technology. [Methods] The UPLC method was established by employing an ACQUITY UPLC®BEH C18 (2.1×100 mm, 1.7 μm) with 0.1% formic acid aqueous solution (A) -acetonitrile (B) as mobile phase in a gradient elution (0~8 min, 3%~8% B; 8~24 min, 8%~19% B; 24~35 min, 19%~45% B). The detection wavelength was 280 nm. The flow rate was 0.3 mL/min and the column temperature was 50 °C. [Results] The established method was employed to develop UPLC-digitized fingerprinting (UPLC-DFP) of multiple dosage forms of Xuefu Zhuyu decoction. By choosing rhoifolin as reference, 30 common peaks were obtained. 16 of 30 common peaks of Xuefu Zhuyu decoction were identified by comparing with standards. The similarity of the multiple dosage forms of Xuefu Zhuyu decoction is over 0.81, which was evaluated by Similarity Evaluation System for Chromatographic Fingerprint of TCM (Version 2004A). The relative peak area of common peaks as characteristic value was implemented to perform principal component analysis (PCA). The multiple dosage forms of Xuefu Zhuyu decoction were classified as three clusters based on different quality. [Conclusion] This method was simple, and provided the basis for the quality control of multiple dosage forms of Xuefu Zhuyu decoction.关键词
血府逐瘀汤/数字化指纹图谱/主成分分析Key words
Xuefu Zhuyu decoction/UPLC-digitized fingerprinting/principal component analysis分类
医药卫生引用本文复制引用
杨龙,杨帆,杨欣莹,张蕾,于卉娟,王跃飞..血府逐瘀汤不同剂型数字化指纹图谱的构建[J].天津中医药,2016,33(9):559-564,6.基金项目
国家自然科学基金青年基金项目（81202877）。 （）
