土壤2016，Vol.48Issue(4)：686-691,6.DOI:10.13758/j.cnki.tr.2016.04.010
红壤水稻土累积酶活性及养分对长期不同施肥处理的响应
Activities of Extracellular Enzymes and Nutrients in Red Paddy Soil Response to Long Term Fertilizations
摘要
Abstract
Based on 24-year long-term field experiment established in 1990 in Yingtan Red Soil Ecological Experiment Station, the experiment included 4 treatments: CK(without fertilization), C(manure), NPK(N,P,K fertilizers), NPKC(NPK plus manure), soil samples were collected. The activities of invertase, urease (adding 0.5 ml toluene and not) and invertase kinetics were determined, and the soil nutrient contents and microbial biomass carbon were measured, in order to analyze the relationships among enzyme activity, soil nutrient and microbial biomass carbon to disclose the response of the activity of extracellular enzyme and nutrients to the long term fertilizations. Compared with CK, application of fertilizers increased the invertase activities by 31.3%–131.7% and microbial biomass carbon by 84.9%–125.1%. Without toluene inhibiting the microorganism activity, the invert rate of the substrate of invertase and urease significantly increased by 89.5%–153.7% and by 59.2%–98.9%, respectively; Microbial biomass carbon had significant positive correlations with the activities of invertase and urease, which imply microorganism significantly influenced the activities of these enzymes. Compared with CK, application of fertilizers increased significantly organic matter (by 30.1%–36.3%), total phosphorus(by 28.6%–102.9%), available phosphorus (by 62.2%–445.0%) and available nitrogen (by 35.9%–56.4%), the activities of soil urease and invertase were positively correlated with the contents of available nitrogen and organic matter. Compared with CK, application of fertilizers increased significantly the transfer coefficient (Vmax/Km) and maximum velocity (Vmax) but influenced little on the Michaelis constant (Km). Long term fertilization increased the soil nutrient contents and microbial biomass carbon, which result in the increase of the activity of the extracellular enzymes.关键词
转化酶/脲酶/微生物生物量碳/土壤酶动力学Key words
Invertase/Urease/Microbial biomass carbon/Enzyme kinetics分类
农业科技引用本文复制引用
李委涛,李忠佩,刘明,江春玉,吴萌..红壤水稻土累积酶活性及养分对长期不同施肥处理的响应[J].土壤,2016,48(4):686-691,6.基金项目
国家自然科学基金项目(41171233，41001143)资助。 （41171233，41001143）
