现代医药卫生2016，Vol.32Issue(18)：2793-2795,2799,4.DOI:10.3969/j.issn.1009-5519.2016.18.004
硫酸右旋糖苷对人胃癌细胞腹腔种植转移防治作用的对比研究
Comparative study of prevention and treatment effect of dextran sulfate on human gastric cancer cells celiac planting ;metastasis
摘要
Abstract
Objective To study the effect of dextran sulfate (DS) between preventive use and treatment use on human gastric cancer cells celiac plantation. Methods Gastric cancer BGC-823 cells were cultured for constructing the nude mice model. One hundred and ten BALB/C nude mice were randomly divided into the prevention group (60 cases) and treatment group (50 cases). The prevention group was simultaneously injected by tumor cells and DS ,while the treatment group was injected with DS at 24 h after injecting tumor cells. The abdominal cavity of nude mice was opened for observing the celiac planting number of gastric cancer cells. The whole nodules were verified by the HE staining. The expression level of greater omentum in-tegrinβ1 protein was detected by immunohistochemistry and the expression of greater omentum integrin β1 mRNA was detected by reverse transcription-polymerase chain reaction(RT-PCR). Results The HE staining showed the number of intraperitoneal metastatic tumor nodules in the treatment group was significantly more than that of the prevention group ,the difference was sta-tistically significant(P<0.01). The immunohistochemical results showed that the expression of integrinβ1 protein in the treatment group was significantly higher than that in the prevention group,the difference was statistically significant(P<0.05). The RT-PCR results showed that the expression of integrin β1 mRNA in the treatment group was significantly higher than that in the preven-tion group,the difference was statistically significant(P<0.01). Conclusion DS has better effect for preventing gastric cancer celiac implanting metastasis than for treating gastric cancer celiac implanting metastasis.关键词
硫酸类/右旋糖酐类/胃肿瘤/病理学/腹腔/抗原,CD29/整合素β1/种植Key words
Sulfuric acids/Dextrans/Stomach neoplasms/pathology/Abdominal cavity/Antigens,CD29/In-tegrin beta 1/Plant引用本文复制引用
王娟,黄允宁,颜贝,王红红,金秀,王潇飞,徐远义..硫酸右旋糖苷对人胃癌细胞腹腔种植转移防治作用的对比研究[J].现代医药卫生,2016,32(18):2793-2795,2799,4.基金项目
宁夏回族自治区科技厅科技支撑项目（2002310201）；宁夏医科大学校级课题项目（XM2015002）。 （）
