心脑血管病防治2016，Vol.16Issue(4)：259-263,5.DOI:10.3969/j.issn.1009-816x.2016.04.05
L 精氨酸和天然抗氧化剂对缺血性脑卒中防治作用的细胞和动物模型研究
Preventive and Therapeutic Effects of L Arginine and Natural Antioxidants on Ischemic Stroke in Cell and Animal Models
赵亚硕 1李砚超 2杜立波 1赵保路 3刘扬 4常彦忠5
作者信息
- 1. 050016 河北师范大学生命科学学院
- 2. 050020 河北中医学院科技处科研中心
- 3. 100190 中国科学院化学研究所
- 4. 100190 中国科学院化学研究所
- 5. 100101 中国科学院，生物物理研究所
- 折叠
摘要
Abstract
Objective To study the preventive and therapeutic effects of L Arginine (L Arg) and natural antioxidants on ischemic stroke .Methods The model of oxygen and glucose deprivation (OGD) on cell and the model of Middle Cerebral Artery Occlusion (MCAO) on mice were established to simulate ischemic stroke .Reactive oxygen species (ROS) levels ,apoptosis and signaling molecules in cell were detected respectively by a pretreatment with L Arg and natural antioxidants .Results In vitro ,a low concentra-tion of L Arg showed no effect on the cell vitality ,but a high level of L Arg could impair the cell and affect the expression of Bcl 2 and Bax .A reasonable combination of L Arg and natural antioxidants could decrease OGD induced cell damage and provide obvious protection on cell vitality by scavenging reactive oxygen species (ROS) ,reducing apoptosis and mitochondrial membrane as well as modulating the expression of related proteins Bcl 2 ,Bax and caspase 3 .In the study of the MCAO model on mice ,we further discov-ered that this combination of L Arg and natural antioxidants could decrease damage and the apoptosis of brain tissue ,and modulate the expression of related proteins Bcl 2 and Bax induced by the focal brain ischemia .Conclusions Since a reasonable combination of L Arg and natural antioxidants can reduce the damage of nerve cells and animal brain tissue induced by hypoxia and ischemia ,it might be beneficial to the prevention and treatment of ischemic stroke .关键词
一氧化氮/天然抗氧化剂/氧糖剥夺/缺血性脑卒中/细胞凋亡Key words
Nitric oxide (NO)/Natural antioxidants/Oxygen and glucose deprivation (OGD)/Ischemia stroke/Apoptosis分类
医药卫生引用本文复制引用
赵亚硕,李砚超,杜立波,赵保路,刘扬,常彦忠..L 精氨酸和天然抗氧化剂对缺血性脑卒中防治作用的细胞和动物模型研究[J].心脑血管病防治,2016,16(4):259-263,5.
