中国病理生理杂志2016，Vol.32Issue(9)：1589-1593,5.DOI:10.3969/j.issn.1000-4718.2016.09.010
Shp2在肺腺癌细胞 A549中的抑癌作用及机制研究
Anticancer function of Shp2 in lung adenocarcinoma A549 cells and rela-ted molecular mechanisms
摘要
Abstract
AIM: To explore the anticancer function of Shp2 in lung adenocarcinoma A549 cells and the related molecular mechanisms.METHODS: The viability and proliferation of A549 cells treated with Shp2 specific inhibi-tor Phps-1 or cisplatin (DDP) were measured by CCK-8 assay and EdU assay.Annexin V-FITC/PI double staining was ap-plied to detect apoptotic rate of A549 cells with different interventions.The protein levels of caspase-3-17p, Bcl-2, Bax, p-STAT3 /STAT3 and p-ERK/ERK were determined by Western blot.RESULTS: Compared with control group, Phps-1 at the concentration of 20 μmol/L significantly increased the viability of A549 cells after 24 h of treatment ( P <0.05). Meanwhile, the proliferation rate of A549 cells in Phps-1 20 μmol/L group was significant increased compared with control group (P <0.05).The apoptotic rate of A549 cells in DDP treatment group decreased from 13.01% ±2.62% to 3.67%±0.93% after adding Phps-1 (P <0.05).Phps-1 down-regulated the protein levels of caspase-3-17p, Bax and p-ERK, but up-regulated p-STAT3.CONCLUSION: Shp2 is a tumor suppressor in A549 cells, which may be associated with the activation of STAT3 signal pathway.关键词
Shp2/肺癌/细胞凋亡Key words
Shp2/Lung cancer/Apoptosis分类
医药卫生引用本文复制引用
陆志伟,程玉生,赵春阳,王寒黎..Shp2在肺腺癌细胞 A549中的抑癌作用及机制研究[J].中国病理生理杂志,2016,32(9):1589-1593,5.基金项目
皖南医学院引进人才基金资助项目（No．YR201107）；皖南医学院中青年基金资助项目 （）
