中国病理生理杂志2016，Vol.32Issue(9)：1608-1613,6.DOI:10.3969/j.issn.1000-4718.2016.09.013
坏死性凋亡介导高糖引起的人脐静脉内皮细胞损伤
Necroptosis mediates high glucose-induced injury in human umbilical vein endothelial cells
摘要
Abstract
AIM: To explore whether necroptosis contributes to the high glucose (HG)-induced damage in hu-man umbilical vein endothelial cells (HUVECs).METHODS: The protein levels of receptor-interacting protein 3 (RIP3) and cleaved caspase-3 were detected by Western blot.The intracellular levels of reactive oxygen species (ROS) were deter-mined by DCFH-DA staining followed by photofluorography.Mitochondrial membrane potential (MMP) was measured by rhodamine 123 staining followed by photofluorography.RESULTS: Treatment of HUVECs with HG at different concentra-tions (10, 20 and 40 mmol/L glucose) for 24 h gradually enhanced the expression levels of RIP3.Treatment of HUVECs with HG (40 mmol/L glucose) for different time (3 h, 6 h, 9 h, 12 h and 24 h) also up-regulated the expression levels of RIP3, peaking at 9 h.Pretreatment of HUVECs with 20 μmol/L Z-VAD-FMK (an inhibitor of caspase) for 30 min before exposure to HG enhanced the expression level of RIP3.Pretreatment of HUVECs with 100 μmol/L necrostatin-1 (an inhi-bitor of necroptosis) for 1 h before exposure to HG alleviated the HG-induced injuries, such as a decrease in cell viability, an increase in ROS generation and dissipation of MMP, but up-regulated the protein level of cleaved caspase-3.CON- <br> CLUSION: Necroptosis mediates HG-induced injury in HUVECs.There is a negative interacting between necroptosis and apoptosis.关键词
坏死性凋亡/细胞凋亡/高糖/人脐静脉内皮细胞/Necrostatin-1Key words
Necroptosis/Apoptosis/High glucose/HUVECs/Necrostatin-1分类
医药卫生引用本文复制引用
林佳琼,陈美姬,郭瑞鲜,张伟杰,智喜梅,邓海鸥,许翎,黎映兰,吴文..坏死性凋亡介导高糖引起的人脐静脉内皮细胞损伤[J].中国病理生理杂志,2016,32(9):1608-1613,6.基金项目
国家自然科学基金资助项目（No．81450062）；广东省自然科学基金资助项目 （）
