中国病理生理杂志2016，Vol.32Issue(9)：1660-1665,6.DOI:10.3969/j.issn.1000-4718.2016.09.021
小檗碱抑制脓毒症诱导的肠上皮细胞凋亡
Berberine inhibits enterocyte apoptosis in septic mice
摘要
Abstract
AIM: To observe the effects of berberine (Ber) on enterocyte apoptosis in septic mice and its pos-sible mechanism.METHODS: Male C57BL/6 mice (8 ~10 weeks old) were randomly divided into sham group, cecal ligation and puncture (CLP) group, CLP +Ber group and sham +Ber group.The mice in CLP group underwent CLP ope-ration, and the mice in sham groups suffered a similar operation except the ligation and puncture.After the sham or CLP operation, the mice were administered intragastrically with distilled water or berberine (50 mg/kg) within 2 h.After 20 h, the mice were killed with excess pentobarbital sodium and the ileum tissues were removed.The histological changes of the intestine were observed and the enterocyte apoptosis was examined by determining the protein level of cleaved caspase-3. Furthermore, mitochondrial Bax, cytoplasm cytochrome C (Cyt C) and the total proteins of Bcl-2, Fas, FasL and Fas-as-sociated protein with death domain (FADD) were examined by Western blot.The mRNA expression of tyrosine hydroxylase (TH) and dopamine beta-hydroxylase (DBH) was measured by real-time PCR.RESULTS: The extensive ileum injuries, including remarkably increased leukocytes and necrosis of intestinal villus were observed 20 h after CLP.In CLP group, the protein levels of cleaved caspase-3, cytoplasm Cyt C, as well as Fas, FasL were significantly increased, but the Bcl-2 level was decreased.Bax translocation into mitochondria was promoted.However, FADD was not changed significantly.The mRNA expression of TH and DBH was also increased sharply in CLP group.On the contrary, treatment with berberine made a considerable alleviating alteration in the ileum of the septic mice.CONCLUSION: Treatment with berberine pro-vides protective effects on intestinal injury in septic mice by reducing enterocyte apoptosis, and its possible mechanism may be involved in the inhibition of the endogenous and exogenous apoptosis pathways.关键词
小檗碱/脓毒症/肠/细胞凋亡/去甲肾上腺素Key words
Berberine/Sepsis/Intestine/Apoptosis/Norepinephrine分类
医药卫生引用本文复制引用
李红梅,邢云,唐翔诩,杨多猛,王华东,吕秀秀,戚仁斌,陆大祥..小檗碱抑制脓毒症诱导的肠上皮细胞凋亡[J].中国病理生理杂志,2016,32(9):1660-1665,6.基金项目
广东省医学科研基金 （）
