中国病理生理杂志2016，Vol.32Issue(9)：1704-1707,1712,5.DOI:10.3969/j.issn.1000-4718.2016.09.029
病毒性心肌炎小鼠心肌中 MMP－2和 NF－κB 的表达
Expression of MMP-2 and NF-κB in myocardium of mice with viral myo-carditis
摘要
Abstract
[ ABSTRACT] AIM: To observe the effects of TNF-α/nuclear factor-κB( NF-κB) /matrix metalloproteinase-2 (MMP-2) pathway on the expression of MMP-2 in the mice with viral myocarditis.METHODS: Six-week-old inbred male mice were randomly assigned to control and myocarditis group.The mice in myocarditis group and control group were intra-peritoneally inoculated with 0.1 mL 10-5.69 TCID50 /mL coxsackievirus B3 and vehicle (PBS), respectively.Ten mice were sacrificed at the 4th and 10th days after injection.The blood and heart specimens were harvested.The serum content of TNF-αwas measured by ELISA.The myocardial levels of MMP-2, NF-κB p65 and IκBαwere determined by Western blot.RESULTS: Compared with control group, the protein expression of MMP-2 and NF-κB p65 in the myocardium and the serum content of TNF-αwere significantly increased in myocarditis group (P <0.05).The protein expression of IκBαwas lower in myocarditis group than that in control group (P <0.05).CONCLUSION: TNF-α, NF-κB p65 and MMP-2 were higher in the mice with acute viral myocarditis.The increased expression of them might be involved in the pathogene-sis of viral myocarditis.关键词
心肌炎/基质金属蛋白酶2/核因 κBKey words
Myocarditis/Matrix metalloproteinase-2/Nuclear factor-κB分类
医药卫生引用本文复制引用
阮妙华,王凯,王丹,周爱华,褚茂平,陈其,钱燕..病毒性心肌炎小鼠心肌中 MMP－2和 NF－κB 的表达[J].中国病理生理杂志,2016,32(9):1704-1707,1712,5.基金项目
温州市科技局基金项目 （）
