中国当代儿科杂志2016，Vol.18Issue(9)：802-805,4.DOI:10.7499/j.issn.1008-8830.2016.09.003
内蒙古西部地区汉族新生儿呼吸窘迫综合征与SP-B外显子7区域R236C位点的相关性研究
Relationship between R236C site in exon 7 of SP-B gene and respiratory distress syndrome in Han newborns in western Inner Mongolia
摘要
Abstract
ObjectiveTo detect and analyze the genetic variation in exon 7 of lung surfactant protein B (SP-B), and to investigate the relationship between the genetic variation and the incidence of neonatal respiratory distress syndrome (NRDS) in Han populations in western Inner Mongolia.MethodsIn the case-control study, 47 Han infants with NRDS were assigned to case group. All the 47 patients had the last three generations of their ancestors reside in western Inner Mongolia. Forty-seven Han newborns without NRDS were assigned to control group. PCR-based gene analysis was used to determine the mutation in exon 7 of SP-B gene and genotype and allele frequencies of the R236C site in exon 7 of SP-B gene.ResultsIn Han newborns in western Inner Mongolia, there was no mutation in exon 7 of SP-B gene; two genotypes, CC and CT, were identiifed in the R236C site in exon 7 of SP-B gene. No TT genotype was found in the two groups. There were no signiifcant differences in the genotype frequency of CC or CT as well as the allele frequency of C or T between the case and control groups (CC: 72% vs 85%,P>0.05; CT: 28% vs 15%,P>0.05;C: 85% vs 93%,P>0.05; T: 15% vs 7%,P>0.05).ConclusionsThere is no mutation in exon 7 of SP-B gene in Han infants with NRDS in western Inner Mongolia. There is no signiifcant association between the gene polymorphism of the R236C site in exon 7 of SP-B gene and the incidence of NRDS in Han populations in that region.关键词
呼吸窘迫综合征/肺表面活性蛋白B/基因多态性/新生儿Key words
Respiratory distress syndrome/Surfactant protein B/Gene polymorphism/Newborn引用本文复制引用
王晶,梅花,刘春枝,张亚昱,刘春丽,宋丹,张钰恒..内蒙古西部地区汉族新生儿呼吸窘迫综合征与SP-B外显子7区域R236C位点的相关性研究[J].中国当代儿科杂志,2016,18(9):802-805,4.基金项目
国家自然科学基金项目（81260107）；内蒙古自然科学基金（2011MS1111）。 （）
