中国动物检疫2016，Vol.33Issue(9)：29-34,6.DOI:10.3969/j.issn.1005-944X.2016.00.000
H5亚型高致病性禽流感病毒抗原性变异分析
Analysis on Antigenic Variation of H5 Subtype Highly Pathogenic Avian Inlfuenza Virus
摘要
Abstract
To further understand the situation of antigenic variation of H5 subtype highly pathogenic avian influenza viruses and its regularity,in this paper,the HA genes of H5 subtype highly pathogenic avian influenza viruses isolated from 2012 to 2015 were amplified,sequenced and analyzed with genetic evolution. It was showed that the epidemic strains were mainly distributed into the clades 2.3.2.1,7.2,and 2.3.4.4. The single factor positive sera against pan-demic strains were prepared and cross hemagglutination inhibition tests were done with standard positive sera against corresponding vaccines. The relativity of antigen index was calculated and the differences of major antigen sites in HA1 genes between popular strains and corresponding vaccine strains were analyzed. The results showed that the relativity of hemagglutination inhibition antigen index of Re-4,Re-5,Re-6,Re-7,Re-8 was between 0.17~0.67,indicated that the antigenicities between the different clades had big differences. With the passage of time,the relativity of hemag-glutination inhibition antigen between popular strains and vaccine strains in one clade will be more and more low,the degree of antigenic variation will increase. It was consistent with the results of variance analysis of the main antigen sites in HA1 region. In order to obtain ideal control effect,vaccines must be updated timely,so as to make them are more married with popular strains. These data will provide technical support for understanding the antigenic variation and the prevention and control of this virus.关键词
H5/禽流感病毒/抗原性/变异/疫苗Key words
H5/avian influenza virus/antigenicity/variation/vaccine分类
农业科技引用本文复制引用
蒋文明,李金平,侯广宇,彭程,刘朔,王素春,陈继明..H5亚型高致病性禽流感病毒抗原性变异分析[J].中国动物检疫,2016,33(9):29-34,6.基金项目
青岛市科技计划（14-2-4-105-jch）；中国动物卫生与流行病学中心创新基金 （）
