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γ-干扰素ELISA检测方法在牛结核病流行病学调查中的应用

吴亚文 王晓亮 张玉玲 王玉梅 李志红 张学军

中国动物检疫2016,Vol.33Issue(9):80-82,3.
中国动物检疫2016,Vol.33Issue(9):80-82,3.DOI:10.3969/j.issn.1005-944X.2016.00.000

γ-干扰素ELISA检测方法在牛结核病流行病学调查中的应用

Applications of IFN-γELISA for Epidemiological Survey of Bovine Tuberculosis

吴亚文 1王晓亮 1张玉玲 1王玉梅 1李志红 1张学军1

作者信息

  • 1. 宁夏动物疾病预防控制中心,宁夏银川 750011
  • 折叠

摘要

Abstract

Objective] In order to investigate the prevalence status of bovine tuberculosis in Helan county and to pro-vide a reference for purification and comprehensive prevention and control of bovine tuberculosis in Ningxia. [Methods] Using the tuberculin skin test(TST)and IFN-γELISA,2 230 cows from 2 large-scale dairy farms were detected. [Results] 79 positive cows from 2 dairy farms were detected by TST,72 of them were still positive results tested by IFN-γELISA subsequently,the double positive rate by both methods was 3.23%(72/2230). [Conclusion] The re-sults showed that the accuracy of bovine tuberculosis IFN-γELISA was relatively higher. TST conjunction with IFN-γELISA could be more conducive to bovine tuberculosis detection and purification.

关键词

牛结核病/γ-干扰素ELISA/皮内变态反应/流行病学调查

Key words

bovine tuberculosis/INF-γELISA/tubulin skin test/epidemiological survey

分类

农业科技

引用本文复制引用

吴亚文,王晓亮,张玉玲,王玉梅,李志红,张学军..γ-干扰素ELISA检测方法在牛结核病流行病学调查中的应用[J].中国动物检疫,2016,33(9):80-82,3.

基金项目

宁夏回族自治区科技支撑项目 ()

中国动物检疫

1005-944X

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