中国康复理论与实践2016，Vol.22Issue(9)：993-998,6.DOI:10.3969/j.issn.1006-9771.2016.09.001
电针对缺血再灌注大鼠脑内神经干细胞分化的影响
Effects of Electroacupuncture on Differentiation of Neural Stem Cells after Cerebral Ischemia-reperfusion in Rats
摘要
Abstract
Objective To explore the effect of electroacupuncture at Quchi (LI11), Zusanli (ST36) on differentiation of neural stem cells after cerebral ischemia-reperfusion in rats. Methods Thirty-six male Sprague-Dawley rats were randomly divided into sham group (n=12), model group (n=12) and electroacupuncture group (n=12). The latter two groups were occluded the left middle cerebral arteries for 90 min-utes and reperfused. The electroacupuncture group received electroacupuncture at Quchi and Zusanli acupoints for 21 days. They were evalu-ated with modified Neurological Severity Scores 7, 14 and 21 days after electroacupuncture. Their infarct volumes were tested with MRI T2WI 21 days after electroacupuncture, while the differentiation of neural stem cells was observed with double-immunopositive BrdU/Dcx and BrdU/NeuN. Results Compared with the model group, the neurological deficits score improved in the electroacupuncture group in all the time points (P<0.05). The infarct volumes decreased in the electroacupuncture group (P<0.05), with less number of BrdU+/Dcx+cells in subventricular zone (P<0.001) and more number of BrdU+/NeuN+ cells in peri-infarct cortex (P<0.001) 21 days after electroacupuncture. Conclusion Electroacupuncture at Quchi and Zusanli acupoints can improve neurological function and decrease the infarct volumes in rats after cerebral ischemia-reperfusion, which may be associated with promoting differentiation of neural stem cells to neurons.关键词
脑缺血再灌注/电针/神经干细胞/分化/大鼠Key words
cerebral ischemia-reperfusion/electroacupuncture/neural stem cell/differentiation/rats分类
医药卫生引用本文复制引用
王露露,林云娇,吴洁,叶晓倩,黄佳,柳维林,陶静,陈立典..电针对缺血再灌注大鼠脑内神经干细胞分化的影响[J].中国康复理论与实践,2016,22(9):993-998,6.基金项目
1.国家自然科学基金项目(No.81373778；No.81273835)；2.福建省卫生厅青年科研课题(No.2014-1-70)。 （No.81373778；No.81273835）
