中国康复理论与实践2016，Vol.22Issue(9)：1020-1023,4.DOI:10.3969/j.issn.1006-9771.2016.09.007
脉冲射频对坐骨神经慢性压迫模型大鼠的痛觉过敏及脊髓背角NR2B亚基表达的作用
Effects of Pulsed Radiofrequency on Hyperalgesia and Expression of NR2B Subunit in Spinal Dorsal Horn in Chronic Constriction Injury Rats
摘要
Abstract
Objective To observe the behavioral and the histopathology changes and expression of NR2B subunit in spinal dorsal horn in chronic constriction injury (CCI) rats after pulsed radiofrequency (PRF). Methods Forty-eight adult Sprague-Dawley rats were randomly divided into Sham-Sham (SS), Sham-PRF (SP), CCI-Sham (CS) and CCI-PRF (CP) groups. The right sciatic nerves (SNs) of the CS and CP groups were ligated to create the CCI model. For the SS and SP groups, the right SNs were separated without ligation. The CP and SP groups accepted PRF at the ligation site 15 days after modeling, while the electrode was placed in rats in the SS and CS groups without elec-tricity. The hindpaw withdrawal threshold (HWT) was measured before and 3, 7, 11, 15 days after modeling, and 1, 3, 7, 11, 15 days after treatment. The right SNs at ligation sites were assessed with optical microscopic score 15 days after treatment, and the NR2B expression in the L4-6 spinal dorsal horn were determined with Western blotting. Results HWT was significantly shorter in the CS and CP groups than in the SS and SP groups after modeling, and was more in the CP group than in the CS group. Under the optical microscope, the axonal diame-ter and myelin sheath thickness increased in the CP group compared to those in the CS group (P<0.01), the NR2B expression was less in the CP group than in the CS group after treatment (F=10.769, P<0.05). Conclusion PRF may reduce hyperalgesia and repair damaged SNs in CCI-induced neuropathic pain, which may associate with inhibition of the NR2B subunit expression in spinal dorsal horn.关键词
神经病理性疼痛/脉冲射频/坐骨神经/痛觉过敏/NR2B亚基Key words
neuropathic pain/pulsed radiofrequency/sciatic nerves/hyperalgesia/NR2B subunit分类
医药卫生引用本文复制引用
王云霞,付淼,罗芳..脉冲射频对坐骨神经慢性压迫模型大鼠的痛觉过敏及脊髓背角NR2B亚基表达的作用[J].中国康复理论与实践,2016,22(9):1020-1023,4.基金项目
北京市卫生系统高层次人才(学科骨干)项目(No.2014-3-035)。 （学科骨干）
