中国免疫学杂志2016，Vol.32Issue(9)：1262-1267,6.DOI:10.3969/j.issn.1000-484X.2016.09.004
HLA-DQB1等位基因多态性及Th1／Th2细胞相关因子与广西瑶族肝癌家族聚集性的相关性
Relationship between HLA-DQB1 allele as well as expression level of Th1/Th2 cytokines with familial clustering of hepatocellular carcinoma in Guangxi yao
摘要
Abstract
Objective:To elucidate the relationship between HLA-DQB1 allele polymorphism as well as the expression level of Th1/Th2 cytokines with familial clustering of hepatocellular carcinoma ( HCC) to provide some evidence for the seeking susceptibility gene or resistant gene of HCC in Guangxi yao,China.Methods:With the same sexuality,age ±5 year,40 members whose families have had two or more HCC patients( high-occurrence families) were selected as the case group,and 40 members whose families have no any cancer patient were selected as the controls.Peripheral blood samples were collected to extract DNA,PCR-SSP was used to detect HLA-DQB1 alleles and ELISA was used to detect IL-2,IL-4 and IL-10.Results:(1) The gene frequency of the HLA-DQB1*02/09 alleles in the case group was higher than that in the controls(P<0.05);but the gene frequency of the HLA-DQB1*04/05/06/07/08 alleles were never significant difference between two groups ( P>0.05 ).( 2 ) The gene frequency of alleles HLA-DQB1 in HBsAg positive group and HBsAg negative group were never significant difference (P>0.05).(3)The expression levels of IL-4,IL-10 in the case group was higher than that in the control ( P<0.05 ).( 4 ) The expression level of IL-10 in the positive group of the HLA-DQB1*02 allele was higher than that in the negative group of the HLA-DQB1*02 allele ( P<0.05 ).( 5 ) The expression level of IL-4 in the positive group of the HLA-DQB1*09 allele was higher than that in the negative group of the HLA-DQB1*09 allele( P<0.05) .Con-clusion:(1) HLA-DQB1*02/09 seem to be susceptibility genes of hepatocellular carcinoma in high HCC incidence areas of Guangxi yao.(2) There may be not significant correlation bewteen HLA-DQB1 alleles and the susceptibility of HBV infection in high HCC incidence areas of Guangxi yao.( 3 ) The imbalance of IL-4, IL-10 might be associated with familial clustering of hepatocellular carcinoma in Guangxi yao.(4)The imbalance of IL-10 might be due to the carrying of HLA-DQB1*02;the imbalance of IL-4 might be due to the carrying of HLA-DQB1*09.Through the same approaches,these might lead to the phenomenon of familial aggregation of HCC in Guangxiyao.关键词
HLA-DQB1/Th1/Th2细胞相关因子/原发性肝癌/家族聚集性Key words
HLA-DQB1/Th1/Th2 cytokines/Hepatocellular carcinoma/Familial clustering分类
医药卫生引用本文复制引用
卢庭婷,梁惠萍,李致忠,黄兰,吴继周,陈婉玲..HLA-DQB1等位基因多态性及Th1／Th2细胞相关因子与广西瑶族肝癌家族聚集性的相关性[J].中国免疫学杂志,2016,32(9):1262-1267,6.基金项目
本文受国家自然科学基金项目（30960170）、广西高校科学技术研究项目（YB2014546）和广西教育厅课题（KY2016YB740）资助。 （）
