中国全科医学2016，Vol.19Issue(27)：3281-3285,3286,6.DOI:10.3969/j.issn.1007-9572.2016.27.005
c - junN 端蛋白激酶1磷酸化与糖尿病大鼠早期心肌损伤关系研究
Relationship between c-junN Terminal Kinase 1 and Early Injury in the Myocardium of Diabetic Rats
摘要
Abstract
Objective To investigate the relationship between c - jun N terminal kinase 1(JNK1)and early injury in the diabetic myocardium( DM)rats. Methods From October 2013 to July 2014,after one - week adaptive feed of the 80 female Sprague - Dawley(SD)rats at SPF levels,60 rats were selected by random number table method. They were established as DM model group by four - week high glucose and high fat diet and a one - time left lower quadrant intraperitoneal injection of streptozotocin(STZ)at a dose of 35 mg/ kg. The other 20 rats,one - time intraperitoneally injected of citrate buffer solution of same volume,were regarded as the control group(group A)with four - week normal diet. In the end,modeling of the 48 rats was established successfully. They were divided into group B,group C and group D with 16 rats in each group by random number table method. Rats in group C were given an intraperitoneal injection of SP600125,rats in group D were subcutaneously implanted with an osmotic pump to infuse angiotensin Ⅱ(Ang Ⅱ)and 0. 9% sodium chlo - ride,rats in group A and group B were given an intraperitoneal injection of 0. 9% sodium chlo - ride. After six - week feeding,10 rats were selected from each group through random number table method. The heart/ body weight ratio(H/ B)and left ventricular mass index(LVMI)were detected. The Hematoxylin and Eosin staining were used to observe the myocardial pathological changes. Immunohistochemistry SABC method was used to measure the expression level of JNK1,phosphorylation JNK1( p - JNK1),transient receptor potential canonical channels(TRPC)6 and collagen Ⅰ. Results The H/ B in group B,group C and group D was higher than that in group A,and LVMI in group B and group D was higher than that in group A(P < 0. 05);H/ B and LVMI of rats in group C were lower than those in group B and group D(P < 0. 05). The myocardial cells of rats in group A arranged in neat rows with tight junctions and clear myocardial texture;while the myocardial cells of rats in group B,group C and group D had hypertrophic changes of different degrees,characterized by dilated intercellular space,disordered cell arrangement and less clear myocardial texture. The expression level of JNK1,p - JNK1,TRPC6,and collagen Ⅰ of rats in group B,group C and group D was higher than that in group A(P < 0. 05);the expression level of JNK1,p - JNK1,TRPC6,and collagen Ⅰ of rats in group C was lower that in group B(P < 0. 05);the expression level of p - JNK1,TRPC6,and collagen Ⅰ of rats in group D was higher than that in group B and group C,the expression level of JNK1 in group D was higher than that in group C(P < 0. 05). Conclusion High glucose environment may induce JNK1 phosphorylation,and affect the expression of TRPC6,which will lead to an increased collagen deposition,and finally result in cardiomyocyte hypertrophy and fibrosis,and cause the occurrence and development of diabetic cardiomyopathy.关键词
糖尿病/心肌疾病/c-junN端蛋白激酶1/瞬时受体电位通道C亚族Key words
Diabetes mellitus/Cardiomyopathies/c - junN terminal kinase 1/Transient receptor potential canonical channels分类
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陈昕明,刘晓亮,吴伟..c - junN 端蛋白激酶1磷酸化与糖尿病大鼠早期心肌损伤关系研究[J].中国全科医学,2016,19(27):3281-3285,3286,6.基金项目
全国临床医药研究专项基金（L201257）；辽宁省科技厅科学技术计划项目 （）
