中国全科医学2016，Vol.19Issue(27)：3287-3290,4.DOI:10.3969/j.issn.1007-9572.2016.27.006
Ⅱ型胶原诱导性关节炎大鼠血清抗角蛋白抗体和抗环瓜氨酸肽抗体及类风湿因子的检测分析
Detection Analysis of Serum Anti - Keratin Antibody,Anti - Cyclic Citrullinated Peptide Antibody and Rheumatoid Factor in Rats with Type Ⅱ Collagen-induced Arthritis
摘要
Abstract
Objective To detect serum anti - keratin antibody( AKA ), anti - cyclic citrullinated peptide (Anti-CCP)antibody and rheumatoid factor(RF)in rats type Ⅱ collagen - induced arthritis(CIA),and to analyze whether they are similar with serum immunology of human rheumatoid arthritis(RA). Methods After one - week adaptive feed,among 15 healthy female Wistar rats(SPF level)aged 7 to 8 weeks,5 were selected as blank control group by random number table method,and 10 for building type Ⅱ CIA model from November 2014 to March 2015,and finally 8 rats' modeling was successfully built(CIA model group). After successful modeling,femoral artery blood of rats in CIA model group and blank control group was collected,and serum was routinely separated,serum AKA of rats in two groups and Anti-CCP antibody and RF - IgM antibody were detected by indirect immunofluorescence(IIF)method and enzyme - linked immunosorbent assay(ELISA) method respectively;40 g/ L paraformaldehyde fixation was used as pathological specimens to isolate synovium of knee joint. The pathological specimens were conducted with HE staining. Results Serum AKA of 5 cases(5 / 8)were positive in CIA model group,all were negative in blank control group. There was no significant difference in levels of serum Anti-CCP antibody and RF- IgM antibody between rats in blank control group and CIA model group(P > 0. 05). Pathological HE staining observation of joint synovium of rats in blank control group presented clear arrangement of synovial cells,normal histology structure,no hyperplasia,no significant accumulation and infiltration of inflammatory cells;pathological HE staining observation of joint synovium of rats in CIA model group showed disordered arrangement of synovial cells,capillary proliferation,significant synovial hyperplasia,synovial infiltration of large amount of inflammatory cells. Conclusion Serum of CIA rats group in shows positive AKA,which is similar with serum immunology of human RA,and suits for basic research of RA.关键词
关节炎,类风湿/大鼠,Wistar/类风湿因子/抗角蛋白抗体/抗环瓜氨酸肽抗体Key words
Arthritis,rheumatoid/Rats,Wistar/Rheumatoid factor/Anti - keratin antibody/Anti - cyclic citrullinated peptide antibody分类
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罗德梅,罗莉,李文红,王倩,杜文静,孟岩,李慧君,闫怡,冯宁,马秀敏..Ⅱ型胶原诱导性关节炎大鼠血清抗角蛋白抗体和抗环瓜氨酸肽抗体及类风湿因子的检测分析[J].中国全科医学,2016,19(27):3287-3290,4.基金项目
新疆维吾尔自治区自然科学基金资助项目 （）
