中国全科医学2016，Vol.19Issue(27)：3317-3321,5.DOI:10.3969/j.issn.1007-9572.2016.27.012
启膈散对食管癌细胞间同质黏附力的影响
Effect of Qigesan on the Cell -cell Adhesion of Esophageal Cancer
摘要
Abstract
Background The incidence of esophageal cancer has distinct geographical characteristics, with approximately 50% of all newly diagnosed cases occurring in China annually. Objective To investigate the effects and mechanism of Qigesan on the cell - cell adhesion of esophageal carcinoma. Methods From March to June in 2015,the human esophageal carcinoma cell lines Eca109,TE13 and TE1 were cultured in vitro. Eca109,TE13 and TE1 were treated with the different dose of Qigesan(0 μg/ ml in control group,50 μg/ ml in low dose group,100 μg/ ml in high dose group),the cell - cell adhesion was tested by mechanical method,the expression level of Cx32 in Eca109,TE13 and TE1 was detected by Western blotting method. The expression of E - cadherin in Eca109,TE13 and TE1 with different dose of Qigesan(0 μg/ ml in control group,100μg/ ml in Qigesan group)was detected by SP immunohistochemistry method. The metastatic abilities of Eca109,TE13 and TE1 with different dose of Qigesan(0 μg/ ml in control group,50 μg/ ml in low dose group,100 μg/ ml in high dose group)were detected by scratch - wound assays. Results The cell - cell adhesion of Eca109,TE13 and TE1 in low dose group and high dose group was higher than that in control group respectively(P < 0. 05);the cell - cell adhesion of Eca109,TE13 and TE1 in high dose group was higher than that in low dose group(P < 0. 05). The expression level of Cx32 of TE1 in low dose group was higher than that in control group(P < 0. 05);the expression level of Cx32 of Eca109,TE13 and TE1 in high dose group was higher than that in control group and low dose group respectively(P < 0. 05). The expression of the E - cadherin was negative in control group and Qigesan group. At 12 h and 24 h,the scratch of Eca109,TE13 and TE1 in low dose group and high dose group was wider than that in control group respectively(P < 0. 05). At 12 h,the scratch of Eca109 in high dose group was wider than that in low dose group(P < 0. 05). At 12 h and 24 h,the scratch of TE13 and TE1 in high dose group was wider than that in low dose group(P < 0. 05). Conclusion Qigesan can improve the expression level of Cx32,then improve the cell - cell adhesive ability and reduce the migratory ability of esophageal carcinoma cells.关键词
食管肿瘤/细胞黏附/细胞运动/启膈散Key words
Esophageal neoplasms/Cell adhesion/Cell movement/Qigesan分类
医药卫生引用本文复制引用
史会娟,高静,石冬璇,李晶..启膈散对食管癌细胞间同质黏附力的影响[J].中国全科医学,2016,19(27):3317-3321,5.基金项目
国家自然科学基金资助项目（81403310）；河北省科技厅资助项目 （）
