中国畜牧兽医2016，Vol.43Issue(9)：2215-2222,8.DOI:10.16431/j.cnki.1671-7236.2016.09.001
JSRV-Env 重组质粒诱导转染 TC-1、TC-1-Hyal2细胞后对 TGF-α及 VEGF 因子表达的影响
JSRV-Env Recombinant Plasmid Induced-transfection of TC-1 and TC-1-Hyal2 and Influence of Cytokine VEGF and TGF-α
摘要
Abstract
JSRV-Env recombinant plasmid was transiently transfected with mouse lung epithelial cell (TC-1 )and ovine Hyal-2 which could stablely express in TC-1 cell (TC-1-Hyal2 )by the method of liposome,then we could explore the mRNA and protein expression of TGF-α and VEGF in order to analyze the correlation TGF-α and VEGF with ovine pulmonary adenomatosis (OPA)and what roles they played in the carcinogenesis process.Both cells were cultured in vitro ,according to different plasmids,TC-1 and TC-1-Hyal2 cells were set pEGFP-C1-env trans-fected groups,pEGFP-C1 transfected and non-transfected groups.The expression of TGF-α and VEGF were detected through Real-time quantitative PCR and ELISA.The PCR results showed that compared with control groups (pEGFP-C1 and non-transfected cells groups),the mRNA expression of VEGF was significantly increased in two cell groups of both transfected with pEGFPC1- env (P <0.05),while the mRNA expression of TGF-αwas extremely significantly increased (P <0.01).The ELISA test results showed that compared with two control groups,the protein expression of VEGF and TGF-αwere extremely significantly increased in TC-1-Hyal2 cell which transfected with pEGFP-C1-env (P <0.01),and in TC-1 cell which transfected with pEGFP-C1- env,the expression of VEGF protein was extremely significantly increased (P <0.01),the protein expression of TGF-αwas significantly increased (P <0.05).There was no significant difference in mRNA and protein expression of two cytokines between pEGFP-C1-transfected and non-transfected cells groups (P >0.05).The results showed that the expression of TGF-αand VEGF both increased in two cell lines which transfected with JSRV-Env.The results suggested JSRV-Env might raise the expression of TGF-αand VEGF,futher we could assume that the TGF-α,VEGF expression changes had a correlation with OPA,and it could provide an important theoretical basis on OPA for gene targeting therapy.关键词
JSRV-Env 重组质粒/TC-1 细胞/TC-1-Hyal2 细胞/TGF-α因子/VEGF 因子Key words
JSRV-Env recombinant plasmid/TC-1 cells/TC-1-Hyal2 cells/TGF-αfactor/VEGF factor分类
生物科学引用本文复制引用
骆爽,孙丽红,斯日古楞,么宏强..JSRV-Env 重组质粒诱导转染 TC-1、TC-1-Hyal2细胞后对 TGF-α及 VEGF 因子表达的影响[J].中国畜牧兽医,2016,43(9):2215-2222,8.基金项目
国家自然科学基金项目(31260593) （31260593）
