中国畜牧兽医2016，Vol.43Issue(9)：2223-2229,7.DOI:10.16431/j.cnki.1671-7236.2016.09.002
陆川猪 PTTG1基因克隆及序列分析
Cloning and Sequence Analysis of PTTG1 Gene in Luchuan Pig
摘要
Abstract
This experiment was aimed to clone PTTG1 gene CDS sequence of Luchuan pig,and was analyzed by bioinformatics methods.A pair of special primers was designed according to pre-dicted sequence of porcine PTTG1 in GenBank.The coding sequence of PTTG1 in Luchuan pig was amplified by RT-PCR,its gene sequence characteristics and protein structure was systemically analyzed by bioinformatics techniques.The results showed that the cloned PTTG1 fragment in-cluded a 609 bp CDS (coding 202 amino acids).The sequence multi-aligned results showed that Luchuan pig shared 90.15%,87.85%,87.52%,87.03%,76.03%,74.38%,55.74% and 44.48%of similar nucleotide sequence with that of Bos ,Pan troglodytes ,Homos ,Macaca ,Rattus ,Mus , Gallus and Danio retio ,respectively.The protein structure analysis results showed that the pro-tein attributed hydrophilic protein without signal peptide,localized in cell cytoplasm and had 1 6 phosphorylation sites.The phylogentic tree of amino acid indicated that PTTG1 was highly con-served in the process of evolution of different species.The cloning and analysis of PTTG1 gene provided an important foundation for further study biological function of porcine PTTG1 during early embryonic development.关键词
陆川猪/PTTG1 基因/克隆/序列分析Key words
Luchuan pig/PTTG1 gene/cloning/sequence analysis分类
生物科学引用本文复制引用
覃兆鲜,农素群,关志惠,梁旦,覃倩,蓝晞,潘天彪,谢炳坤..陆川猪 PTTG1基因克隆及序列分析[J].中国畜牧兽医,2016,43(9):2223-2229,7.基金项目
国家自然科学基金(31360544) （31360544）
广西自然科学基金(2013GXNSFBA019117) （2013GXNSFBA019117）
广西科学研究与技术开发计划项目(桂科重14121003-2-2) （桂科重14121003-2-2）
广西水产畜牧科技推广应用项目(桂渔牧科201452020) （桂渔牧科201452020）
