中国畜牧兽医2016，Vol.43Issue(9)：2240-2248,9.DOI:10.16431/j.cnki.1671-7236.2016.09.004
DNA 甲基化酶抑制剂5-Aza-CdR 对多能基因及甲基化相关基因在徒手克隆胚胎中表达模式的影响
Effect of DNA Methylation Inhibitor 5-Aza-CdR on Patterns of Pluripotent-related Genes and Methylation Genes in Handmade Cloning Embryo
摘要
Abstract
The expression pattern of pluripotent gene Oct4 and Nanog ,and methylation related genes Dnmt 1 and Tets of handmade cloning (HMC)embryos were studied by Real-time quantita-tive PCR assay.The effect of genes expression pattern by 5-Aza-CdR on HMC reconstructed em-bryos was also explored.The results showed that the expression of Oct4,Nanog and Tet3 genes reached peak on 2-cell stage,the expression of Dnmt 1 and Tet2 genes declined with the embryo development,while the expression of Tet 1 gene increased.The use of 5-Aza-CdR didn’t change the expression pattern of Oct4,Tet 1 and Tet3 genes,but increased the expression of Nanog gene at the beginning of embryo development,while decreased the expression of Dnmt 1 and Tet2 genes.The expression pattern of Oct4,Nanog ,Dnmt 1 and Tets genes in the development of HMC embryo was established,the use of 5-Aza-CdR could influence the methylation process of HMC embryo.关键词
徒手克隆/5-Aza-CdR/基因/表达模式Key words
HMC/5-Aza-CdR/gene/expression pattern分类
农业科技引用本文复制引用
陆杏蓉,李志鹏,孙俊铭,吕玲燕,许钟峯,刘庆友,石德顺,崔奎青..DNA 甲基化酶抑制剂5-Aza-CdR 对多能基因及甲基化相关基因在徒手克隆胚胎中表达模式的影响[J].中国畜牧兽医,2016,43(9):2240-2248,9.基金项目
国家自然基金(31260552) （31260552）
国家高技术研究发展计划(863)项目(2011AA100607) （863）
