中国畜牧兽医2016，Vol.43Issue(9)：2249-2256,8.DOI:10.16431/j.cnki.1671-7236.2016.09.005
猪繁殖与呼吸综合征病毒 FS 株 Nsp 9基因的克隆与序列分析
Cloning and Sequence Analysis of Nsp9 Gene of Porcine Reproductive and Respiratory Syndrome Virus FS Strain
摘要
Abstract
In order to evaluate the function of Nsp 9 gene,the target gene of porcine reproductive and respiratory syndrome virus (PRRSV)FS strain was amplificated and sequenced after being cloned into the pMD18-T vector.The physical and chemical properties,homology,hydrophilicity, surface probability plot,antigenic index,secondary structure and subcellular localization were pre-dicted by various softwares.The results showed that the length of Nsp9 was 1 929 bp,its predic-ted molecular weight was 70.5 ku and pI was 7.78,and it was unstable protein.There were many antigen sites,and the flexibility and hydrophilicity of Nsp9 were ideal.The study showed that Nsp9 possessed potential antigenicity,and it fits for preparation of monoclonal antibodies.The re-sults of subcellular location showed that it might exist in the cytoplasm.Nsp9 of FS strain shared 96.9% to 98.9% amino acid homology with other strains of the some hypotype.Some amino acid mutations were found between the parent strains and vaccine strains,and insertions and deletions could be found among the European strains and the American strains.Whether these insertions and deletions correlate with the virulence and polymerase needed further research.关键词
猪繁殖与呼吸综合征病毒/Nsp 9 基因/克隆/序列分析Key words
porcine reproductive and respiratory syndrome virus/Nsp 9 gene/clone/sequence analysis分类
生物科学引用本文复制引用
赵孟孟,宋中宝,冯松林,王文佳,邢星,冯嘉萍,张桂红..猪繁殖与呼吸综合征病毒 FS 株 Nsp 9基因的克隆与序列分析[J].中国畜牧兽医,2016,43(9):2249-2256,8.基金项目
国家自然科学基金猪繁殖与呼吸综合征病毒非结构蛋白 Nsp9功能研究项目(31272564) （31272564）
公益性行业(农业)科研专项经费(201203039) （农业）
国家生猪现代农业产业技术体系(CARS-36)项目 （CARS-36）
国家重点研发计划课题(2016YFD0500707) （2016YFD0500707）
