中国畜牧兽医2016，Vol.43Issue(9)：2257-2264,8.DOI:10.16431/j.cnki.1671-7236.2016.09.06
哈萨克马和焉耆马不同组织中 IGFBP-5基因表达差异研究
Study on Expression Difference of IGFBP-5 Gene in Different Tissues of Kazakh and Yanqi Horses
摘要
Abstract
The study aimed to explore the mRNA expression pattern of insulin-like growth factor binding protein-5 (IGFBP-5)gene in different tissues of Kazakh and Yanqi horses.The expression of IGFBP-5 gene in different tissues of heart,liver,spleen,lung,kidney,small intestine,large in-testine,cecum,intercostal muscles,longissimus dorsi muscles,brachialis muscle and gluteus in two horses were detected by Real-time quantitative PCR and compared the mRNA expression in the same tissues of two breeds.The results showed that the expression of IGFBP-5 in longissimus dorsi muscle and brachialis muscle of two breeds were significantly higher than other tissues in-cluding heart,liver,spleen,lung,kidney,small intestine,large intestine and cecum (P <0.05),and was the lowest in large intestine.The expression of IGFBP-5 in kidney,small intestine,large in-testine,cecum,longissimus dorsi muscle and brachialis muscles of Yanqi horse were higher than in the same part of Kazakh horse,and among those in longissimus dorsi muscle and large intestine of Yanqi horse were extremely significantly higher than in the same part of Kazakh horse (P <0.01),and in small intestine and cecum of Yanqi horse were significantly higher than in the same part of Kazakh horse (P <0.05).The test was for further researching the biological function of IGFBP-5 gene,and it could provide a theoretical basis for genetic improvement of production per-formance of horse in our country.关键词
IGFBP-5 基因/哈萨克马/焉耆马/mRNA 表达/实时荧光定量 PCRKey words
IGFBP-5 gene/Kazakh horse/Yanqi horse/mRNA expression/Real-time quantitative PCR分类
生物科学引用本文复制引用
吐尔逊江·吾木尔艾力,黄晶晶,阿米娜,孟军,王建文,曾亚琦,姚新奎,李林玲,姚芳芳,王欢,任海帆..哈萨克马和焉耆马不同组织中 IGFBP-5基因表达差异研究[J].中国畜牧兽医,2016,43(9):2257-2264,8.基金项目
自治区科技厅重大专项《马(运动、肉用、乳用)生产关键技术研究与示范》(201130101) （运动、肉用、乳用）
国家科技支撑计划项目《优质运动马培育生产关键技术研究与集成示范》(2011BAD28B06) （2011BAD28B06）
国家科技支撑计划"速步、乳用、耐力马新品种(系)培育及良种扩繁技术研究与集成示范"(2012BAD44B00) （系）
